Proteomic Study of Retinal Proteins Associated with Transcorneal Electric Stimulation in Rats

Takashi Kanamoto; Nazariy Souchelnytskyi; Takuji Kurimoto; Yasuhiro Ikeda; Hiroaki Sakaue; Yasunari Munemasa; Yoshiaki Kiuchi

doi:10.1155/2015/492050

Journal of Ophthalmology (Jan 2015)

Proteomic Study of Retinal Proteins Associated with Transcorneal Electric Stimulation in Rats

Takashi Kanamoto,
Nazariy Souchelnytskyi,
Takuji Kurimoto,
Yasuhiro Ikeda,
Hiroaki Sakaue,
Yasunari Munemasa,
Yoshiaki Kiuchi

Affiliations

Takashi Kanamoto: Department of Ophtahlomology, Hiroshima Memorial Hospital, 1-4-3 Honkawacho, Minami-ku, Hiroshima 730-0802, Japan
Nazariy Souchelnytskyi: Personalized Cancer Medicine Unit, Department of Oncology-Pathology, Karolinska Institute, 171 77 Stockholm, Sweden
Takuji Kurimoto: Department of Ophthalmology, Osaka Medical College, 2-7 Daigaku-cho, Takatsuki-shi, Osaka 569-8686, Japan
Yasuhiro Ikeda: Department of Ophthalmology, Kyushu University, 3-1-1 Maedashi, Higashi-ku, Fukuoka 812-8592, Japan
Hiroaki Sakaue: Department of Pharmaceutical Sciences, International University of Health and Welfare, 2600-1 Kitakanemaru, Tahara-shi, Tochigi 324-8501, Japan
Yasunari Munemasa: Department of Ophthalmology, St. Marianna Medical College, 2-16-1 Sugao, Miyamae-ku, Kawasaki-shi, Kanagawa 216-8511, Japan
Yoshiaki Kiuchi: Department of Ophthalmology and Visual Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshiam 734-8551, Japan

DOI: https://doi.org/10.1155/2015/492050
Journal volume & issue: Vol. 2015

Abstract

Read online

Background. To investigate how transcorneal electric stimulation (TES) affects the retina, by identifying those proteins up- and downregulated by transcorneal electric stimulation (TES) in the retina of rats. Methods. Adult Wistar rats received TES on the left eyes at different electrical currents while the right eyes received no treatment and served as controls. After TES, the eye was enucleated and the retina was isolated. The retinas were analyzed by proteomics. Results. Proteomics showed that twenty-five proteins were upregulated by TES. The identified proteins included cellular signaling proteins, proteins associated with neuronal transmission, metabolic proteins, immunological factors, and structural proteins. Conclusions. TES induced changes in expression of various functional proteins in the retina.

Published in Journal of Ophthalmology

ISSN: 2090-004X (Print); 2090-0058 (Online)
Publisher: Wiley
Country of publisher: United Kingdom
LCC subjects: Medicine: Ophthalmology
Website: https://onlinelibrary.wiley.com/journal/3846

About the journal