Construction of AC/DC magnetic syringe device for stimulated drug release, injection and ejection of nanocarriers and testing cytotoxicity in vitro
Milorad Zjalić,
Mislav Mustapić,
Zvonko Glumac,
Ivan Prološčić,
Senka Blažetić,
Ana Vuković,
Mostafa Masud,
Motasim Billah,
Aslam Khan,
Suzana Šegota,
Md Shahriar Al Hossain,
Marija Heffer
Affiliations
Milorad Zjalić
Department of Medical Biology and Genetics, Faculty of Medicine, J.J. Strossmayer University of Osijek, J. Huttlera 4, 31 000 Osijek, Croatia
Mislav Mustapić
Department of Physics, J.J. Strossmayer University of Osijek, Trg Ljudevita Gaja 6, Osijek 31000, Croatia
Zvonko Glumac
Department of Physics, J.J. Strossmayer University of Osijek, Trg Ljudevita Gaja 6, Osijek 31000, Croatia
Ivan Prološčić
Department of Physics, J.J. Strossmayer University of Osijek, Trg Ljudevita Gaja 6, Osijek 31000, Croatia
Senka Blažetić
Department of Biology, J.J. Strossmayer University of Osijek, Ulica cara Hadrijana 8a, Osijek 31000, Croatia
Ana Vuković
Department of Biology, J.J. Strossmayer University of Osijek, Ulica cara Hadrijana 8a, Osijek 31000, Croatia
Mostafa Masud
Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St. Lucia, Brisbane QLD 4067, Australia
Motasim Billah
Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St. Lucia, Brisbane QLD 4067, Australia; School of Mechanical and Mining Engineering, University of Queensland, St. Lucia, Brisbane QLD 4067, Australia
Aslam Khan
King Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11451, Saudi Arabia
Suzana Šegota
Division of Physical Chemistry, Ruđer Bošković Institute, Zagreb 10000, Croatia
Md Shahriar Al Hossain
Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St. Lucia, Brisbane QLD 4067, Australia; School of Mechanical and Mining Engineering, University of Queensland, St. Lucia, Brisbane QLD 4067, Australia
Marija Heffer
Department of Medical Biology and Genetics, Faculty of Medicine, J.J. Strossmayer University of Osijek, J. Huttlera 4, 31 000 Osijek, Croatia; Corresponding author.
Iron nanoparticles are used as a targeted drug delivery system. The nanocarrier itself can be genotoxic, trigger oxidative stress or cell death. Therefore, we developed an AC/DC magnetic syringe for injecting, stimulating drug release and safe removing of the nanocarrier. Alongside we optimized the method for nanoparticles’ drug release kinetics and testing cytotoxicity in vitro. • This paper presents detailed instructions for construction of AC/DC magnetic syringe device for stimulated drug release, injection and ejection of magnetic nanoparticles; nanoparticles preparation; adsorbing methylene blue on nanoparticles; determination of drug release kinetics from nanocarriers on the example of methylene blue • Gomori´s Prussian blue reaction for differentiated SH-SY5Y human neuroblastoma cell line; MTT viability assay optimized for differentiated SH-SY5Y human neuroblastoma cell line and antioxidant enzymes activities assay and lipid peroxidation methods are optimized for cell analyses cell cultivation for nanoparticles cytotoxicity testing in vitro. • Those protocols are the first step toward further testing the effect of nanoparticles in vivo, on brain tissue.
