Nature Communications (Feb 2021)

A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry

  • Yunkai Zhu,
  • Fei Feng,
  • Gaowei Hu,
  • Yuyan Wang,
  • Yin Yu,
  • Yuanfei Zhu,
  • Wei Xu,
  • Xia Cai,
  • Zhiping Sun,
  • Wendong Han,
  • Rong Ye,
  • Di Qu,
  • Qiang Ding,
  • Xinxin Huang,
  • Hongjun Chen,
  • Wei Xu,
  • Youhua Xie,
  • Qiliang Cai,
  • Zhenghong Yuan,
  • Rong Zhang

DOI
https://doi.org/10.1038/s41467-021-21213-4
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 11

Abstract

Read online

The SARS-CoV-2 spike protein contains a multi-basic cleavage site. Here, the authors show how this multi-basic cleavage site affects entry of SARS-CoV-2 into cells and transmission in the hamster model and identify host factors affecting entry of SARS-CoV-2 in a genome-wide CRISPR screen.