Frontiers in Immunology (Oct 2022)

The IgG glycome of SARS-CoV-2 infected individuals reflects disease course and severity

  • Sterre L. Siekman,
  • Tamas Pongracz,
  • Wenjun Wang,
  • Jan Nouta,
  • Peter G. Kremsner,
  • Peter G. Kremsner,
  • Peter G. Kremsner,
  • Pedro Vieira da Silva-Neto,
  • Meral Esen,
  • Meral Esen,
  • Meral Esen,
  • Andrea Kreidenweiss,
  • Andrea Kreidenweiss,
  • Andrea Kreidenweiss,
  • Jana Held,
  • Jana Held,
  • Jana Held,
  • Átila Alexandre Trapé,
  • Rolf Fendel,
  • Rolf Fendel,
  • Rolf Fendel,
  • Isabel Kinney Ferreira de Miranda Santos,
  • Manfred Wuhrer,
  • ImmunoCovid Consortium,
  • Alessandro P. de Amorim,
  • Jamille G M. Argolo,
  • Rita de C.C. Barbieri,
  • Marcelo Dias-Baruffi,
  • Victor A F. Bastos,
  • Vânia L D. Bonat,
  • Cristina Ribeiro de Barros Cardoso,
  • Ingryd Carmona-Garcia,
  • Jonatan C S de Carvalho,
  • Leticia F. Constant,
  • Augusto M. Degiovani,
  • Cassia F.S.L. Dias,
  • Lúcia H. Faccioli,
  • Marley R. Feitosa,
  • Omar Feres,
  • Ana Paula Morais Fernandes,
  • Talita M. Fernandes,
  • Thais F C. Fraga-Silva,
  • Carlos Fuzo,
  • Isabelle C. Guarneri,
  • Cristiane M. Milanezi,
  • Caroline T. Garbato,
  • Gilberto Gambero Gaspar,
  • Ângelo A.F. Júnior,
  • Sandra R. Maruyama,
  • Debora C. Nepomuceno,
  • Nicola T. Neto,
  • Camilla Narjara Simão Oliveira,
  • Fátima M. Ostini,
  • Rogerio S. Parra,
  • Malena M. Pérez,
  • Vinı́cius E. Pimentel,
  • Giovanna da S. Porcel,
  • José J R da Rocha,
  • Lilian C. Rodrigues,
  • Elisa M S. Russo,
  • Dayane P. da Silva,
  • Rafael C. da Silva,
  • Carlos Arterio Sorgi,
  • Camila O S. Souza,
  • Diana M. Toro,
  • Angelina L. Viana,
  • Fernando Crivelenti Vilar,
  • Ana C. Xavier,
  • Kamila Zaparoli

DOI
https://doi.org/10.3389/fimmu.2022.993354
Journal volume & issue
Vol. 13

Abstract

Read online

Immunoglobulin G (IgG) antibodies play an important role in the immune response against viruses such as SARS-CoV-2. As the effector functions of IgG are modulated by N-glycosylation of the Fc region, the structure and possible function of the IgG N-glycome has been under investigation in relation to divergent COVID-19 disease courses. Through LC-MS analysis we studied both total IgG1 and spike protein-specific IgG1 Fc glycosylation of 129 German and 163 Brazilian COVID-19 patients representing diverse patient populations. We found that hospitalized COVID-19 patients displayed decreased levels of total IgG1 bisection and galactosylation and lowered anti-S IgG1 fucosylation and bisection as compared to mild outpatients. Anti-S IgG1 glycosylation was dynamic over the disease course and both anti-S and total IgG1 glycosylation were correlated to inflammatory markers. Further research is needed to dissect the possible role of altered IgG glycosylation profiles in (dys)regulating the immune response in COVID-19.

Keywords