Plasma-generated RONS in liquid transferred into cryo-microneedles patch for skin treatment of melanoma
Jishen Zhang,
Tong Wu,
Zifeng Wang,
Shengduo Xu,
Xixi Jing,
Zizhu Zhang,
Jiao Lin,
Hao Zhang,
Dingxin Liu,
Renwu Zhou,
Li Guo,
Xiaohua Wang,
Mingzhe Rong,
Yongping Shao,
Kostya (Ken) Ostrikov
Affiliations
Jishen Zhang
State Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi'an Jiaotong University, Xi'an City, 710049, People's Republic of China; Interdisciplinary Research Center of Frontier Science and Technology, Xi'an Jiaotong University, Xi'an City, 710049, People's Republic of China
Tong Wu
State Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi'an Jiaotong University, Xi'an City, 710049, People's Republic of China
Zifeng Wang
State Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi'an Jiaotong University, Xi'an City, 710049, People's Republic of China; Interdisciplinary Research Center of Frontier Science and Technology, Xi'an Jiaotong University, Xi'an City, 710049, People's Republic of China
Shengduo Xu
State Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi'an Jiaotong University, Xi'an City, 710049, People's Republic of China
Xixi Jing
State Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi'an Jiaotong University, Xi'an City, 710049, People's Republic of China
Zizhu Zhang
State Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi'an Jiaotong University, Xi'an City, 710049, People's Republic of China
Jiao Lin
State Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi'an Jiaotong University, Xi'an City, 710049, People's Republic of China
Hao Zhang
State Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi'an Jiaotong University, Xi'an City, 710049, People's Republic of China; Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, People's Republic of China; Interdisciplinary Research Center of Frontier Science and Technology, Xi'an Jiaotong University, Xi'an City, 710049, People's Republic of China; Corresponding author. State Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi'an Jiaotong University, Xi'an City, 710049, People's Republic of China.
Dingxin Liu
State Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi'an Jiaotong University, Xi'an City, 710049, People's Republic of China; Interdisciplinary Research Center of Frontier Science and Technology, Xi'an Jiaotong University, Xi'an City, 710049, People's Republic of China
Renwu Zhou
State Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi'an Jiaotong University, Xi'an City, 710049, People's Republic of China; Interdisciplinary Research Center of Frontier Science and Technology, Xi'an Jiaotong University, Xi'an City, 710049, People's Republic of China
Li Guo
State Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi'an Jiaotong University, Xi'an City, 710049, People's Republic of China; Interdisciplinary Research Center of Frontier Science and Technology, Xi'an Jiaotong University, Xi'an City, 710049, People's Republic of China
Xiaohua Wang
State Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi'an Jiaotong University, Xi'an City, 710049, People's Republic of China
Mingzhe Rong
State Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi'an Jiaotong University, Xi'an City, 710049, People's Republic of China
Yongping Shao
Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, People's Republic of China
Kostya (Ken) Ostrikov
School of Chemistry and Physics and Centre for Biomedical Technologies, Queensland University of Technology (QUT), Brisbane, QLD, 4000, Australia
Malignant melanoma is the most lethal form of skin cancer. As a promising anti-cancer agent, plasma-activated water (PAW) rich in reactive oxygen and nitrogen species (RONS) has shown significant potential for melanoma treatment. However, rapid decay of RONS and inefficient delivery of PAW in conventional injection methods limit its practical applications. To address this issue, here we report a new approach for the production of plasma-activated cryo-microneedles (PA-CMNs) patches using custom-designed plasma devices and processes. Our innovation is to incorporate PAW into the PA-CMNs that are fabricated using a fast cryogenic micro-molding method. It is demonstrated that PA-CMNs can be easily inserted into skin to release RONS and slow the decay of RONS thereby prolonging their bioactivity and effectiveness. The new insights into the effective melanoma treatment suggest that the rich mixture of RONS within PA-CMNs prepared by custom-developed hybrid plasma-assisted configuration induces both ferroptosis and apoptosis to selectively kill tumor cells. A significant inhibition of subcutaneous A375 melanoma growth was observed in PA-CMNs-treated tumor-bearing nude mice without any signs of systemic toxicity. The new approach based on PA-CMNs may potentially open new avenues for a broader range of disease treatments.
