Stem Cell Research (Jul 2017)

Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9

  • Yingying Xu,
  • Yuhang Wu,
  • Dongsheng Guo,
  • Ge Gao,
  • Keyu Lai,
  • Fan Yang,
  • Kepin Wang,
  • Han Wu,
  • Liangxue Lai,
  • Jialiang Li,
  • Kecheng Xu,
  • Yin-xiong Li

DOI
https://doi.org/10.1016/j.scr.2017.05.006
Journal volume & issue
Vol. 22, no. C
pp. 29 – 32

Abstract

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The gene asialoglycoprotein receptor 1 (ASGR1) encodes a subunit of the asialoglycoprotein receptor. Here we report the generation of a human embryonic stem cell line WAe001-A-6 harbouring homozygous ASGR1 mutations using CRISPR/Cas9. The mutation involves a 37 bp deletion, resulting in a frame shift. The homozygous knockout WA01 cell line maintains a normal karyotype, typical stem cell morphology, pluripotency and differentiation potential in vitro.