Determination of two acrylates in environmental water by high performance liquid chromatography

Abstract

A high performance liquid chromatography (HPLC) method was established for the determination of two acrylate substances in environmental water. The optimal chromatographic conditions were determined via exploring the effects of chromatographic column, mobile phase, column temperature, flow rate, detection wavelength and other factors on the separation effect of acrylate substances. Finally, the effective separation of methyl methacrylate and isopropyl methacrylate was realized within 6 min. The retention time of the target compound was used for qualitative analysis and the external standard method was used for quantitative analysis in the experiment. The linear relationship between the two acrylates was good in the range of 0.2-50.0 mg/L, and the correlation coefficient of standard curve was higher than 0.999. The recovery rate was 88.6%-105.3%, the relative standard deviation was 1.7%-4.1%, and the detection limit (LODs) was 0.03-0.05 mg/L. The method was simple, efficient and accurate, and suitable for the determination of acrylates in environmental water samples.