Pathogens (Nov 2021)

Improving Current Knowledge on Seroprevalence and Genetic Characterization of Swine Influenza Virus in Croatian Pig Farms: A Retrospective Study

  • Andreja Jungić,
  • Vladimir Savić,
  • Josip Madić,
  • Ljubo Barbić,
  • Besi Roić,
  • Dragan Brnić,
  • Jelena Prpić,
  • Lorena Jemeršić,
  • Dinko Novosel

DOI
https://doi.org/10.3390/pathogens10111527
Journal volume & issue
Vol. 10, no. 11
p. 1527

Abstract

Read online

In a total of 1536 blood serum samples analysed by ELISA, antibodies for IAV nucleoprotein (NP) were detected in 30.3%. Results from HI show that the most common subtype of swIAV in the Croatian pig population was H1N1 (44.6%), followed by H3N2 (42.7%) and H1N2 (26.3%). Antibodies to at least one subtype were detected in 62.19% of blood serum samples. Detection of swIAV antigen was performed by IHC and detected in 8 of 28 lung samples collected post-mortem. The matrix (M) gene was detected in nine of one hundred and forty-two lung tissue samples and in seven of twenty-nine nasopharyngeal swabs. Phylogenetic analysis of amplified HA and NA gene fragments in Croatian isolates suggests the presence of swIAV H1avN1av.

Keywords