Broad and potently neutralizing monoclonal antibodies isolated from human survivors of New World hantavirus infection

Taylor B. Engdahl; Natalia A. Kuzmina; Adam J. Ronk; Chad E. Mire; Matthew A. Hyde; Nurgun Kose; Matthew D. Josleyn; Rachel E. Sutton; Apoorva Mehta; Rachael M. Wolters; Nicole M. Lloyd; Francisca R. Valdivieso; Thomas G. Ksiazek; Jay W. Hooper; Alexander Bukreyev; James E. Crowe, Jr.

Cell Reports (May 2021)

Broad and potently neutralizing monoclonal antibodies isolated from human survivors of New World hantavirus infection

Taylor B. Engdahl,
Natalia A. Kuzmina,
Adam J. Ronk,
Chad E. Mire,
Matthew A. Hyde,
Nurgun Kose,
Matthew D. Josleyn,
Rachel E. Sutton,
Apoorva Mehta,
Rachael M. Wolters,
Nicole M. Lloyd,
Francisca R. Valdivieso,
Thomas G. Ksiazek,
Jay W. Hooper,
Alexander Bukreyev,
James E. Crowe, Jr.

Affiliations

Taylor B. Engdahl: Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
Natalia A. Kuzmina: Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA; Galveston National Laboratory, Galveston, TX 77550, USA
Adam J. Ronk: Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA; Galveston National Laboratory, Galveston, TX 77550, USA
Chad E. Mire: Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA; Galveston National Laboratory, Galveston, TX 77550, USA; Animal Resource Center, University of Texas Medical Branch, Galveston, TX 77555, USA
Matthew A. Hyde: Animal Resource Center, University of Texas Medical Branch, Galveston, TX 77555, USA
Nurgun Kose: Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA
Matthew D. Josleyn: Virology Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA
Rachel E. Sutton: Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA
Apoorva Mehta: Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA
Rachael M. Wolters: Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
Nicole M. Lloyd: Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA; Galveston National Laboratory, Galveston, TX 77550, USA
Francisca R. Valdivieso: Programa Hantavirus, Instituto de Ciencias e Innovación en Medicina (ICIM), Facultad de Medicina, Clínica Alemana Universidad del Desarrollo, Santiago 7590943, Chile
Thomas G. Ksiazek: Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA; Galveston National Laboratory, Galveston, TX 77550, USA
Jay W. Hooper: Virology Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA
Alexander Bukreyev: Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA; Galveston National Laboratory, Galveston, TX 77550, USA; Corresponding author
James E. Crowe, Jr.: Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Corresponding author

Journal volume & issue: Vol. 35, no. 5
p. 109086

Abstract

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Summary: New World hantaviruses (NWHs) are endemic in North and South America and cause hantavirus cardiopulmonary syndrome (HCPS), with a case fatality rate of up to 40%. Knowledge of the natural humoral immune response to NWH infection is limited. Here, we describe human monoclonal antibodies (mAbs) isolated from individuals previously infected with Sin Nombre virus (SNV) or Andes virus (ANDV). Most SNV-reactive antibodies show broad recognition and cross-neutralization of both New and Old World hantaviruses, while many ANDV-reactive antibodies show activity for ANDV only. mAbs ANDV-44 and SNV-53 compete for binding to a distinct site on the ANDV surface glycoprotein and show potently neutralizing activity to New and Old World hantaviruses. Four mAbs show therapeutic efficacy at clinically relevant doses in hamsters. These studies reveal a convergent and potently neutralizing human antibody response to NWHs and suggest therapeutic potential for human mAbs against HCPS.

Published in Cell Reports

ISSN: 2211-1247 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: http://www.cell.com/cell-reports/home

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