Data in Brief (Mar 2016)
Proteome-wide dataset supporting the study of ancient metazoan macromolecular complexes
- Sadhna Phanse,
- Cuihong Wan,
- Blake Borgeson,
- Fan Tu,
- Kevin Drew,
- Greg Clark,
- Xuejian Xiong,
- Olga Kagan,
- Julian Kwan,
- Alexandr Bezginov,
- Kyle Chessman,
- Swati Pal,
- Graham Cromar,
- Ophelia Papoulas,
- Zuyao Ni,
- Daniel R. Boutz,
- Snejana Stoilova,
- Pierre C. Havugimana,
- Xinghua Guo,
- Ramy H. Malty,
- Mihail Sarov,
- Jack Greenblatt,
- Mohan Babu,
- W. Brent Derry,
- Elisabeth R. Tillier,
- John B. Wallingford,
- John Parkinson,
- Edward M. Marcotte,
- Andrew Emili
Affiliations
- Sadhna Phanse
- Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada
- Cuihong Wan
- Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada; Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA
- Blake Borgeson
- Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada
- Fan Tu
- Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA
- Kevin Drew
- Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA
- Greg Clark
- Department of Medical Biophysics, Toronto, Ontario, Canada
- Xuejian Xiong
- Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada; Hospital for Sick Children, Toronto, Ontario, Canada
- Olga Kagan
- Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada
- Julian Kwan
- Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada; Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
- Alexandr Bezginov
- Department of Medical Biophysics, Toronto, Ontario, Canada
- Kyle Chessman
- Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada; Hospital for Sick Children, Toronto, Ontario, Canada
- Swati Pal
- Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada; Hospital for Sick Children, Toronto, Ontario, Canada
- Graham Cromar
- Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada; Hospital for Sick Children, Toronto, Ontario, Canada
- Ophelia Papoulas
- Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA
- Zuyao Ni
- Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada
- Daniel R. Boutz
- Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA
- Snejana Stoilova
- Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada
- Pierre C. Havugimana
- Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada
- Xinghua Guo
- Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada
- Ramy H. Malty
- Department of Biochemistry, University of Regina, Regina, Saskatchewan, Canada
- Mihail Sarov
- Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
- Jack Greenblatt
- Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada; Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
- Mohan Babu
- Department of Biochemistry, University of Regina, Regina, Saskatchewan, Canada
- W. Brent Derry
- Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada; Hospital for Sick Children, Toronto, Ontario, Canada
- Elisabeth R. Tillier
- Department of Medical Biophysics, Toronto, Ontario, Canada
- John B. Wallingford
- Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA; Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA
- John Parkinson
- Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada; Hospital for Sick Children, Toronto, Ontario, Canada
- Edward M. Marcotte
- Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA; Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA
- Andrew Emili
- Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada; Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada; Corresponding author at: Banting and Best Department of Medical Research, Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto, CCBR, Rm 914, 160 College Street, Toronto, Ontario, Canada M5S 3E1. Tel.: +1 617 610 4042; fax: +1 416 978 8528.
- Journal volume & issue
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Vol. 6
pp. 715 – 721
Abstract
Our analysis examines the conservation of multiprotein complexes among metazoa through use of high resolution biochemical fractionation and precision mass spectrometry applied to soluble cell extracts from 5 representative model organisms Caenorhabditis elegans, Drosophila melanogaster, Mus musculus, Strongylocentrotus purpuratus, and Homo sapiens. The interaction network obtained from the data was validated globally in 4 distant species (Xenopus laevis, Nematostella vectensis, Dictyostelium discoideum, Saccharomyces cerevisiae) and locally by targeted affinity-purification experiments. Here we provide details of our massive set of supporting biochemical fractionation data available via ProteomeXchange (http://www.ebi.ac.uk/pride/archive/projects/PXD002319-http://www.ebi.ac.uk/pride/archive/projects/PXD002328), PPIs via BioGRID (185267); and interaction network projections via (http://metazoa.med.utoronto.ca) made fully accessible to allow further exploration. The datasets here are related to the research article on metazoan macromolecular complexes in Nature [1]. Keywords: Proteomics, Metazoa, Protein complexes, Biochemical, Fractionation
