Anti-Microbial Effect of AgBr-NP@CTMAB on Streptococcus Mutans and Assessment of Surface Roughness Hardness and Flexural Strength of PMMA

Abstract

Jing-Jing Huang,1,* Lin Jia,2,3,* Qiao-Jun Zhang,4 Hao-Hong Li,5 Da-Li Zheng,2 Ming Zheng3 1Second Department of Dental Implant, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, 350001, People’s Republic of China; 2Fujian Key Laboratory of Oral Diseases, Fujian Biological Materials Engineering and Technology Center of Stomatology, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, Fujian, 350004, People’s Republic of China; 3Department of Prosthodontics, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, 350001, People’s Republic of China; 4Zhongshan Hospital Affiliated to Xiamen University, Xiamen, 361000, People’s Republic of China; 5College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, People’s Republic of China*These authors contributed equally to this workCorrespondence: Ming Zheng, Department of Prosthodontics, School and Hospital of Stomatology, Fujian Medical University, 246 Yangqiao Middle Road, Fuzhou, 350001, People’s Republic of China, Tel [phone], Email [email protected] Da-Li Zheng, Fujian Key Laboratory of Oral Diseases, School and Hospital of Stomatology, Fujian Medical University, 88 Jiaotong Road, Fuzhou, 350004, People’s Republic of China, Tel [phone], Email [email protected]: To investigate the inhibition of Streptococcus mutans (S.mutans) and its biofilm by AgBr-nanoparticles (NP) @CTMAB (cetyltrimethyl-ammonium bromide) and evaluate the changes in Polymethyl methacrylate (PMMA)’s surface roughness (Ra), microhardness, and flexural strength during prolonged immersion in AgBr-NP@CTMAB for application in the denture cleaning industry.Patients and Methods: The antibacterial activity of AgBr-NP@CTMAB against S.mutans was measured colony formation assay, OD600 and laser confocal microscopy. Changes in the specimens’ values for surface roughness, microhardness, and flexural strength (MPa) were measured after immersion solutions for 180 or 360 days.Results: The AgBr-NP@CTMAB solution exhibited a robust antibacterial effect on planktonic S. mutans, with a minimum bactericidal concentration of 5 μg/mL. The 10 μg/mL AgBr-NP@CTMAB solution efficiently inhibited S. mutans biofilm formation. (2) No significant difference in surface roughness after immersion in AgBr-NP@CTMAB (10 μg/mL and 20 μg/mL) comparing with distilled water (P > 0.05) and Polident had significantly higher than distilled water (P 0.05). The result of flexural strength suggested that there was no statistically significant difference (P < 0.05) between AgBr-NP@CTMAB as well as Polident and water.Conclusion: AgBrNP@CTMAB can efficiently inhibit the growth of plankton S.mutans and biofilm formation, without affecting the flexural strength, microhardness, or surface roughness of PMMA. Therefore, AgBrNP@CTMAB holds promise as a new denture cleaning agent.Keywords: AgBr-NP@CTMAB, S.mutans, polymethyl methacrylate, denture cleanser, biofilm

Keywords

• agbr-np@ctmab
• s.mutans
• polymethyl methacrylate
• denture cleanser
• biofilm