OsNPF3.1, a member of the NRT1/PTR family, increases nitrogen use efficiency and biomass production in rice
Xinghai Yang,
Baoxuan Nong,
Can Chen,
Junrui Wang,
Xiuzhong Xia,
Zongqiong Zhang,
Yu Wei,
Yu Zeng,
Rui Feng,
Yanyan Wu,
Hui Guo,
Haifeng Yan,
Yuntao Liang,
Shuhui Liang,
Yong Yan,
Danting Li,
Guofu Deng
Affiliations
Xinghai Yang
Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China
Baoxuan Nong
Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China
Can Chen
Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China
Junrui Wang
Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Nanning 530006, Guangxi, China
Xiuzhong Xia
Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China
Zongqiong Zhang
Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China
Yu Wei
Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China
Yu Zeng
Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China
Rui Feng
Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China
Yanyan Wu
Biotechnology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China
Hui Guo
Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China
Haifeng Yan
Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China
Yuntao Liang
Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China
Shuhui Liang
Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China
Yong Yan
Microbiology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China; Corresponding authors.
Danting Li
Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China; Corresponding authors.
Guofu Deng
Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China; Corresponding authors.
The overuse of nitrogen (N) fertilizer in fields has increased production costs and raised environmental concerns. Increasing the N use efficiency (NUE) of rice varieties is crucial for sustainable agriculture. Here we report the cloning and characterization of OsNPF3.1, a gene that controls rice NUE. An amino acid mutation in the OsNPF3.1 coding region caused different NUEs in wild and cultivated rice. OsNPF3.1, which is expressed mainly in the aerial parts of rice, also affects rice plant height, heading date, and thousand-grain weight. The OsNPF3.1 protein is located in the plasma membrane. When OsNPF3.1 was subjected to artificial selection, two naturally varying loci were associated with NUE, of which OsNPF3.1Chr6_8741040 differed between indica and japonica rice. OsNPF3.1 can be used as a new target gene for breeding rice varieties with high NUE.
