Nature Communications (Jan 2024)
Diverse array of neutralizing antibodies elicited upon Spike Ferritin Nanoparticle vaccination in rhesus macaques
- Rajeshwer S. Sankhala,
- Kerri G. Lal,
- Jaime L. Jensen,
- Vincent Dussupt,
- Letzibeth Mendez-Rivera,
- Hongjun Bai,
- Lindsay Wieczorek,
- Sandra V. Mayer,
- Michelle Zemil,
- Danielle A. Wagner,
- Samantha M. Townsley,
- Agnes Hajduczki,
- William C. Chang,
- Wei-Hung Chen,
- Gina C. Donofrio,
- Ningbo Jian,
- Hannah A. D. King,
- Cynthia G. Lorang,
- Elizabeth J. Martinez,
- Phyllis A. Rees,
- Caroline E. Peterson,
- Fabian Schmidt,
- Tricia J. Hart,
- Debra K. Duso,
- Lawrence W. Kummer,
- Sean P. Casey,
- Jazmean K. Williams,
- Shruthi Kannan,
- Bonnie M. Slike,
- Lauren Smith,
- Isabella Swafford,
- Paul V. Thomas,
- Ursula Tran,
- Jeffrey R. Currier,
- Diane L. Bolton,
- Edgar Davidson,
- Benjamin J. Doranz,
- Theodora Hatziioannou,
- Paul D. Bieniasz,
- Dominic Paquin-Proulx,
- William W. Reiley,
- Morgane Rolland,
- Nancy J. Sullivan,
- Sandhya Vasan,
- Natalie D. Collins,
- Kayvon Modjarrad,
- Gregory D. Gromowski,
- Victoria R. Polonis,
- Nelson L. Michael,
- Shelly J. Krebs,
- M. Gordon Joyce
Affiliations
- Rajeshwer S. Sankhala
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- Kerri G. Lal
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- Jaime L. Jensen
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- Vincent Dussupt
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- Letzibeth Mendez-Rivera
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- Hongjun Bai
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- Lindsay Wieczorek
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- Sandra V. Mayer
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- Michelle Zemil
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- Danielle A. Wagner
- Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
- Samantha M. Townsley
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- Agnes Hajduczki
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- William C. Chang
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- Wei-Hung Chen
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- Gina C. Donofrio
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- Ningbo Jian
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- Hannah A. D. King
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- Cynthia G. Lorang
- Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
- Elizabeth J. Martinez
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- Phyllis A. Rees
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- Caroline E. Peterson
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- Fabian Schmidt
- Laboratory of Retrovirology, The Rockefeller University
- Tricia J. Hart
- Trudeau Institute
- Debra K. Duso
- Trudeau Institute
- Lawrence W. Kummer
- Trudeau Institute
- Sean P. Casey
- Trudeau Institute
- Jazmean K. Williams
- Integral Molecular
- Shruthi Kannan
- Integral Molecular
- Bonnie M. Slike
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- Lauren Smith
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- Isabella Swafford
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- Paul V. Thomas
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- Ursula Tran
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- Jeffrey R. Currier
- Viral Diseases Branch, Walter Reed Army Institute of Research
- Diane L. Bolton
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- Edgar Davidson
- Integral Molecular
- Benjamin J. Doranz
- Integral Molecular
- Theodora Hatziioannou
- Laboratory of Retrovirology, The Rockefeller University
- Paul D. Bieniasz
- Laboratory of Retrovirology, The Rockefeller University
- Dominic Paquin-Proulx
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- William W. Reiley
- Trudeau Institute
- Morgane Rolland
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- Nancy J. Sullivan
- Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
- Sandhya Vasan
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- Natalie D. Collins
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- Kayvon Modjarrad
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- Gregory D. Gromowski
- Viral Diseases Branch, Walter Reed Army Institute of Research
- Victoria R. Polonis
- U.S. Military HIV Research Program, Walter Reed Army Institute of Research
- Nelson L. Michael
- Center for Infectious Disease Research, Walter Reed Army Institute of Research
- Shelly J. Krebs
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- M. Gordon Joyce
- Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research
- DOI
- https://doi.org/10.1038/s41467-023-44265-0
- Journal volume & issue
-
Vol. 15,
no. 1
pp. 1 – 19
Abstract
Abstract The repeat emergence of SARS-CoV-2 variants of concern (VoC) with decreased susceptibility to vaccine-elicited antibodies highlights the need to develop next-generation vaccine candidates that confer broad protection. Here we describe the antibody response induced by the SARS-CoV-2 Spike Ferritin Nanoparticle (SpFN) vaccine candidate adjuvanted with the Army Liposomal Formulation including QS21 (ALFQ) in non-human primates. By isolating and characterizing several monoclonal antibodies directed against the Spike Receptor Binding Domain (RBD), N-Terminal Domain (NTD), or the S2 Domain, we define the molecular recognition of vaccine-elicited cross-reactive monoclonal antibodies (mAbs) elicited by SpFN. We identify six neutralizing antibodies with broad sarbecovirus cross-reactivity that recapitulate serum polyclonal antibody responses. In particular, RBD mAb WRAIR-5001 binds to the conserved cryptic region with high affinity to sarbecovirus clades 1 and 2, including Omicron variants, while mAb WRAIR-5021 offers complete protection from B.1.617.2 (Delta) in a murine challenge study. Our data further highlight the ability of SpFN vaccination to stimulate cross-reactive B cells targeting conserved regions of the Spike with activity against SARS CoV-1 and SARS-CoV-2 variants.
