Nature Communications (Apr 2019)
The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells
- Weihua Tian,
- Zilu Ye,
- Shengjun Wang,
- Morten Alder Schulz,
- Julie Van Coillie,
- Lingbo Sun,
- Yen-Hsi Chen,
- Yoshiki Narimatsu,
- Lars Hansen,
- Claus Kristensen,
- Ulla Mandel,
- Eric Paul Bennett,
- Siamak Jabbarzadeh-Tabrizi,
- Raphael Schiffmann,
- Jin-Song Shen,
- Sergey Y. Vakhrushev,
- Henrik Clausen,
- Zhang Yang
Affiliations
- Weihua Tian
- Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen
- Zilu Ye
- Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen
- Shengjun Wang
- Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen
- Morten Alder Schulz
- Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen
- Julie Van Coillie
- Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen
- Lingbo Sun
- Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen
- Yen-Hsi Chen
- Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen
- Yoshiki Narimatsu
- Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen
- Lars Hansen
- Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen
- Claus Kristensen
- GlycoDisplay ApS
- Ulla Mandel
- Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen
- Eric Paul Bennett
- Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen
- Siamak Jabbarzadeh-Tabrizi
- Institute of Metabolic Disease, Baylor Scott & White Research Institute
- Raphael Schiffmann
- Institute of Metabolic Disease, Baylor Scott & White Research Institute
- Jin-Song Shen
- Institute of Metabolic Disease, Baylor Scott & White Research Institute
- Sergey Y. Vakhrushev
- Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen
- Henrik Clausen
- Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen
- Zhang Yang
- Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen
- DOI
- https://doi.org/10.1038/s41467-019-09809-3
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 13
Abstract
Lysosomal replacement enzymes are taken up by cell surface receptors that recognize glycans, the effects of different glycan features are unknown. Here the authors present a gene engineering screen in CHO cells that allows custom N-glycan-decorated enzymes with improved circulation time and organ distribution.
