Membrane Localized GbTMEM214s Participate in Modulating Cotton Resistance to Verticillium Wilt
Jun Zhao,
Jianwen Xu,
Yueping Wang,
Jianguang Liu,
Chengguang Dong,
Liang Zhao,
Nijiang Ai,
Zhenzhen Xu,
Qi Guo,
Guoli Feng,
Peng Xu,
Junling Cheng,
Xin Wang,
Juan Wang,
Songhua Xiao
Affiliations
Jun Zhao
Key Laboratory of Cotton and Rapeseed, Institute of Industrial Crops, Ministry of Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Jianwen Xu
Key Laboratory of Cotton and Rapeseed, Institute of Industrial Crops, Ministry of Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Yueping Wang
Key Laboratory of Cotton and Rapeseed, Institute of Industrial Crops, Ministry of Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Jianguang Liu
Key Laboratory of Cotton and Rapeseed, Institute of Industrial Crops, Ministry of Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Chengguang Dong
Cotton Research Institute, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, China
Liang Zhao
Key Laboratory of Cotton and Rapeseed, Institute of Industrial Crops, Ministry of Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Nijiang Ai
Shihezi Agricultural Science Research Institute, Shihezi 832000, China
Zhenzhen Xu
Key Laboratory of Cotton and Rapeseed, Institute of Industrial Crops, Ministry of Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Qi Guo
Key Laboratory of Cotton and Rapeseed, Institute of Industrial Crops, Ministry of Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Guoli Feng
Shihezi Agricultural Science Research Institute, Shihezi 832000, China
Peng Xu
Key Laboratory of Cotton and Rapeseed, Institute of Industrial Crops, Ministry of Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Junling Cheng
Key Laboratory of Cotton and Rapeseed, Institute of Industrial Crops, Ministry of Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Xin Wang
Cotton Research Institute, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, China
Juan Wang
Cotton Research Institute, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, China
Songhua Xiao
Key Laboratory of Cotton and Rapeseed, Institute of Industrial Crops, Ministry of Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Verticillium wilt (VW) is a soil-borne fungal disease caused by Verticillium dahliae Kleb, which leads to serious damage to cotton production annually in the world. In our previous study, a transmembrane protein 214 protein (TMEM214) gene associated with VW resistance was map-based cloned from Gossypium barbadense (G. barbadense). TMEM214 proteins are a kind of transmembrane protein, but their function in plants is rarely studied. To reveal the function of TMEM214s in VW resistance, all six TMEM214s were cloned from G. barbadense in this study. These genes were named as GbTMEM214-1_A/D, GbTMEM214-4_A/D and GbTMEM214-7_A/D, according to their location on the chromosomes. The encoded proteins are all located on the cell membrane. TMEM214 genes were all induced with Verticillium dahliae inoculation and showed significant differences between resistant and susceptible varieties, but the expression patterns of GbTMEM214s under different hormone treatments were significantly different. Virus-induced gene silencing analysis showed the resistance to VW of GbTMEM214s-silenced lines decreased significantly, which further proves the important role of GbTMEM214s in the resistance to Verticillium dahliae. Our study provides an insight into the involvement of GbTMEM214s in VW resistance, which was helpful to better understand the disease-resistance mechanism of plants.
