The microbiological characteristics of lower respiratory tract infection in patients with neuromuscular disorders: An investigation based on a multiplex polymerase chain reaction to detect viruses and a clone library analysis of the bacterial 16S rRNA gene sequence in sputum samples

Masato Ogawa; Takayuki Hoshina; Kaoru Haro; Tokiko Kumadaki; Masahiro Ishii; Yoshihisa Fujino; Kazumasa Fukuda; Koichi Kusuhara

Journal of Microbiology, Immunology and Infection (Oct 2019)

The microbiological characteristics of lower respiratory tract infection in patients with neuromuscular disorders: An investigation based on a multiplex polymerase chain reaction to detect viruses and a clone library analysis of the bacterial 16S rRNA gene sequence in sputum samples

Masato Ogawa,
Takayuki Hoshina,
Kaoru Haro,
Tokiko Kumadaki,
Masahiro Ishii,
Yoshihisa Fujino,
Kazumasa Fukuda,
Koichi Kusuhara

Affiliations

Masato Ogawa: Department of Pediatrics, School of Medicine, University of Occupational and Environmental Health, Japan
Takayuki Hoshina: Department of Pediatrics, School of Medicine, University of Occupational and Environmental Health, Japan; Corresponding author. Department of Pediatrics, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan. Fax: +81 93 691 9338.
Kaoru Haro: Department of Pediatrics, School of Medicine, University of Occupational and Environmental Health, Japan
Tokiko Kumadaki: Department of Pediatrics, School of Medicine, University of Occupational and Environmental Health, Japan
Masahiro Ishii: Department of Pediatrics, School of Medicine, University of Occupational and Environmental Health, Japan
Yoshihisa Fujino: Department of Preventive Medicine and Community Health, School of Medicine, University of Occupational and Environmental Health, Japan
Kazumasa Fukuda: Department of Microbiology, School of Medicine, University of Occupational and Environmental Health, Japan
Koichi Kusuhara: Department of Pediatrics, School of Medicine, University of Occupational and Environmental Health, Japan

Journal volume & issue: Vol. 52, no. 5
pp. 827 – 830

Abstract

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We performed gene amplification methods for the detections of bacteria and viruses using sputum samples to clarify the microbiological characteristics of lower respiratory tract infection in patients with neuromuscular disorders. The tendencies of higher proportion of respiratory virus detection and lower diversity of bacteria in sputum were observed. Keywords: Neuromuscular disorders, Lower respiratory tract infection, Respiratory viruses, 16S rRNA gene, Microbiome in respiratory tract

Published in Journal of Microbiology, Immunology and Infection

ISSN: 1684-1182 (Print)
Publisher: Elsevier
Country of publisher: Hong Kong
LCC subjects: Science: Microbiology
Website: https://www.sciencedirect.com/journal/journal-of-microbiology-immunology-and-infection

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