Genetic diversity of Gossypium barbadense germplasm resources and identification of resistance to Verticillium wilt

Abstract

Abstract [Objective] Through identification and analysis of disease resistance of the field- and indoorgrowing seedlings, the distribution of disease resistance of Gossypium barbadense germplasm resources was mastered, providing a basis for screening high resistant resources to Verticillium wilt. [Methods] 238 G. barbadense germplasm resources of our research group were used as objects. In 2023, the field plot test for the natural disease was carried out in KEL and AWT, and the resistance of G. barbadense resources to V. wilt was identified and evaluated by indoor inoculation. [Results] The genetic diversity of 15 indexes of 238 G. barbadense germplasm resources was analyzed. The coefficient of variation was ranged from 2.00% to 36.97%, with an average of 16.05%. Resistance identification in different environments showed that the coefficient of variation was ranged from 8.40% to 71.06%. Based on the analysis of four different environments, no germplasm resources with complete immunity and high resistance to V. wilt were identified, and 30 resistant materials, 194 resistant materials, and 14 susceptible materials were identified. [Conclusion] Most of the planting resources of G. barbadense have resistance to V. wilt. Therefore, it is necessary to identify and select germplasm resources with strong resistance to V. wilt from G. barbadense germplasm resources.

Keywords

• germplasm resources
• genetic diversity
• verticillium wilt
• resistance identification
• gossypium barbadense