Antibacterial Hydrogel Sheet Dressings Composed of Poly(vinyl alcohol) and Silver Nanoparticles by Electron Beam Irradiation
Rattanakorn Chiangnoon,
Pennapa Karawak,
Jarurattana Eamsiri,
Sasikarn Nuchdang,
Nuatawan Thamrongsiripak,
Naruemon Neramitmansook,
Siwanut Pummarin,
Pimchanok Pimton,
Kewalee Nilgumhang,
Pimpon Uttayarat
Affiliations
Rattanakorn Chiangnoon
Nuclear Technology Research and Development Center, Thailand Institute of Nuclear Technology (Public Organization), Ongkarak, Nakhon Nayok 26120, Thailand
Pennapa Karawak
Nuclear Technology Research and Development Center, Thailand Institute of Nuclear Technology (Public Organization), Ongkarak, Nakhon Nayok 26120, Thailand
Jarurattana Eamsiri
Nuclear Technology Research and Development Center, Thailand Institute of Nuclear Technology (Public Organization), Ongkarak, Nakhon Nayok 26120, Thailand
Sasikarn Nuchdang
Nuclear Technology Research and Development Center, Thailand Institute of Nuclear Technology (Public Organization), Ongkarak, Nakhon Nayok 26120, Thailand
Nuatawan Thamrongsiripak
Irradiation Center, Thailand Institute of Nuclear Technology (Public Organization), Ongkarak, Nakhon Nayok 26120, Thailand
Naruemon Neramitmansook
Irradiation Center, Thailand Institute of Nuclear Technology (Public Organization), Ongkarak, Nakhon Nayok 26120, Thailand
Siwanut Pummarin
Department of Biology, School of Science, Walailak University, Nakhon Si Thammarat 80160, Thailand
Pimchanok Pimton
Department of Biology, School of Science, Walailak University, Nakhon Si Thammarat 80160, Thailand
Kewalee Nilgumhang
Advanced Engineering and Nuclear Technology Center, Thailand Institute of Nuclear Technology (Public Organization), Ongkarak, Nakhon Nayok 26120, Thailand
Pimpon Uttayarat
Nuclear Technology Research and Development Center, Thailand Institute of Nuclear Technology (Public Organization), Ongkarak, Nakhon Nayok 26120, Thailand
Advanced wound dressings that can deliver potent antibacterial action are still much in need, especially for treating wound infections caused by drug-resistant bacteria. In this research, we utilized electron beam (EB) irradiation to develop antibacterial hydrogel sheet dressings from poly(vinyl alcohol) (PVA) and silver nanoparticles (AgNPs) in a two-step processing and evaluated their bactericidal efficacy, as well as the AgNP release. The effect of the irradiation dose on the swelling, gel fraction, network parameters, and mechanical properties of the hydrogels was first determined to establish the optimal doses for the two-step processing. The prototypic hydrogel sheets were then formed in the first EB irradiation and served as a matrix for the AgNP synthesis by the reduction of the silver nitrate precursors during the second EB irradiation. The diffusion assay showed that the minimal inhibition concentration (MIC) of the AgNP-load hydrogels was 0.25 and 0.5 mg/cm2 against Escherichia coli and Staphylococcus aureus, respectively. At these MIC levels, the released AgNPs increased sharply before reaching the maximum, ~950 and 1800 ppb, at 24 h as analyzed by atomic absorption. Therefore, we successfully demonstrated that this two-step processing by EB irradiation provides a convenient platform to fabricate AgNP-loaded hydrogel dressings that can be further developed for wound healing.
