DIA Comparative Proteomic Analysis of Retro-oil Fluid and Vitreous Fluid From Retinal Detachment Patients

Yiyang Shu; Yiyang Shu; Yiyang Shu; Yiyang Shu; Yiyang Shu; Min Gao; Min Gao; Min Gao; Min Gao; Min Gao; Yifan Zhou; Haiyun Liu; Haiyun Liu; Haiyun Liu; Haiyun Liu; Haiyun Liu; Xiaodong Sun; Xiaodong Sun; Xiaodong Sun; Xiaodong Sun; Xiaodong Sun

doi:10.3389/fmolb.2021.763002

Frontiers in Molecular Biosciences (Dec 2021)

DIA Comparative Proteomic Analysis of Retro-oil Fluid and Vitreous Fluid From Retinal Detachment Patients

Yiyang Shu,
Yiyang Shu,
Yiyang Shu,
Yiyang Shu,
Yiyang Shu,
Min Gao,
Min Gao,
Min Gao,
Min Gao,
Min Gao,
Yifan Zhou,
Haiyun Liu,
Haiyun Liu,
Haiyun Liu,
Haiyun Liu,
Haiyun Liu,
Xiaodong Sun,
Xiaodong Sun,
Xiaodong Sun,
Xiaodong Sun,
Xiaodong Sun

Affiliations

Yiyang Shu: Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Yiyang Shu: National Clinical Research Center for Eye Diseases, Shanghai, China
Yiyang Shu: Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai, China
Yiyang Shu: Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai, China
Yiyang Shu: Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, Shanghai, China
Min Gao: Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Min Gao: National Clinical Research Center for Eye Diseases, Shanghai, China
Min Gao: Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai, China
Min Gao: Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai, China
Min Gao: Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, Shanghai, China
Yifan Zhou: Putuo People’s Hospital, Tongji University, Shanghai, China
Haiyun Liu: Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Haiyun Liu: National Clinical Research Center for Eye Diseases, Shanghai, China
Haiyun Liu: Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai, China
Haiyun Liu: Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai, China
Haiyun Liu: Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, Shanghai, China
Xiaodong Sun: Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Xiaodong Sun: National Clinical Research Center for Eye Diseases, Shanghai, China
Xiaodong Sun: Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai, China
Xiaodong Sun: Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai, China
Xiaodong Sun: Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, Shanghai, China

DOI: https://doi.org/10.3389/fmolb.2021.763002
Journal volume & issue: Vol. 8

Abstract

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Objectives: There have been reports of unexplained visual loss following intra-ocular silicone oil (SiO) tamponade in retinal detachment patients, yet the underlying mechanism is unknown. The aim of this study was to investigate the mechanisms behind retinal toxicity following intra-ocular SiO tamponade in retinal detachment patients.Methods and Results: Vitreous fluid samples were acquired from 27 patients (27 eyes). Twelve eyes for data-independent acquisition (DIA) were divided into four groups: pars plana vitrectomy (PPV) for rhegmatogenous retinal detachment (RD group), SiO removal after successful retinal reattachment (SO group), cataract surgery after successful retinal reattachment with sterilized air tamponade (FA group), and PPV for epiretinal membrane (ERM group). The remaining 15 eyes were used for enzyme-linked immunosorbent assay analysis. DIA was combined with two-dimensional liquid chromatography–tandem mass spectrometry to find expression changes in the proteome of vitreous. Mean number mass spectra, statistically differentially expressed proteins, gene ontology (GO), pathway representations, and protein interactions were analyzed. GO analysis showed that the protein categories of synapse organization, cell adhesion, and regulation of cell migration in the SO group were differentially expressed compared to the control or FA groups (p < 0.05). Through Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, lysosome and cell adhesion were found to be significantly enriched in the SO group compared to the FA and control groups (p < 0.05). Cadherin 2, transferrin, and lysosome function may partially contribute to silicone oil-related vision loss.Conclusion: Vision loss-inducing novel molecular signatures and pathways that may be associated with SiO toxicity were identified. Transferrin may be a potential visual outcome biomarker for SiO tamponade.

Published in Frontiers in Molecular Biosciences

ISSN: 2296-889X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: https://www.frontiersin.org/journals/molecular-biosciences

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