Unveiling the effect of paper matrix on the electrochemical response of diffusive redox probes

Abstract

This study evaluates the influence of paper substrate selection on the electrochemical response of an electrochemical paper-based analytical device (ePAD). Various paper substrates commonly employed in this type of devices such as cellulose, nitrocellulose and glass-fibre based medical grade materials are evaluated using chronoamperometric measurements. Both theoretical modelling and experimental analyses are conducted to understand the diffusion behaviour of widely employed two redox probes, [Fe(CN)6]3− and FcMeOH. Findings show that glass fibre substrates show similar performance to liquid drop conditions while nitrocellulose cause a decrease in current after a short measurement period, mainly due to a thin-layer effect. Cellulose-based substrates decrease the diffusivity of redox species, especially for charged species, indicating potential limitations in their use for chronoamperometric measurements. The study offers valuable insights into the electrochemical behaviour of paper substrates in ePADs, laying the groundwork for future research in this area.

Keywords

• Paper microfluidics
• ePADS
• Simulations