Three genome assemblies of Opsariichthys bidens from Yangzte River, Pearl River and Qiantang River basins

Abstract

Abstract Opsariichthys bidens is an endemic minnow, mainly distributed in China as an emerging aquaculture species. In this study, O. bidens collected from Yangzte River (YR), Pearl River (PR) and Qiantang River (QR) basins were used for karyotypic study and genome sequencing. Using PacBio long reads and Hi-C data, we assembled high-quality O. bidens genomes of 852.41 Mb (YR), 843.11 Mb (PR) and 840.94 Mb (QR) with scaffold N50 lengths of 21.01 Mb, 23.62 Mb and 24.75 Mb, respectively, of which 90.39%, 95.67% and 99.01% were anchored to 37, 38 and 39 chromosomes, respectively. 26,556 (YR), 25,036 (PR) and 26,283 (QR) protein-coding genes were predicted, respectively. The karyotype of O. bidens from YR, PR and QR were 2 N = 74 (6 m + 6sm + 4st + 58t), 2 N = 76 (4 m + 6sm + 4st + 62t) and 2 N = 78 (4 m + 4sm + 4st + 66t), respectively. Collinearity analysis and telomere predictions indicated that the observed chromosomal evolution was driven by Robertsonian translocation. These genome assemblies facilitate cryptic species determination, evolutionary study and genetic breeding of genus Opsariichthys.