MICROPROPAGATION OF YUCCA PLANT BY USING GUAR AND LOCUST BEAN SEED POWDER AS AN ALTERNATIVE CHEAP GELLING AGENT

Abstract

The present study aims to determine the best type and concentration of growth regulators and find cheap and/or readily-available alternative substitutes of agar for tissue culture media preparation. The results clear that MS medium supplemented with 1 mg l-1 BA significantly outperformed other tested cytokinins concerning yucca shoot multiplication and growth parameters (number of shoots/explant, number of leaves/shoot, and shoot length (cm)) which recorded 4.75, 14.00 and 6.50 cm, respectively. Moreover, the combination between 2 g l-1 agar and 6 g l-1 guar as gelling agents produced the maximum shoot growth values. During rooting stage, data clear that 1.0 mg l-1 NAA increased number of leaves/shoot, number of roots/shoot and root length with mean values of 20.50, 3.75 and 5.22 cm, respectively. Finally, rooted plantlets were successfully acclimatized with maximum survivability percentage (100%) in soil mixture of sand and peat moss (1:1. v/v).

Keywords

• yucca
• ba
• gelling agent
• guar seeds powder
• naa