In Vitro and In Vivo Antioxidant and Anti-Hyperglycemic Activities of Moroccan Oat Cultivars
Ilias Marmouzi,
El Mostafa Karym,
Nezha Saidi,
Bouchra Meddah,
Mourad Kharbach,
Azlarab Masrar,
Mounya Bouabdellah,
Layachi Chabraoui,
Khalid El Allali,
Yahia Cherrah,
My El Abbes Faouzi
Affiliations
Ilias Marmouzi
Laboratoire de Pharmacologie et Toxicologie, équipe de Pharmacocinétique, Faculté de Médicine et Pharmacie, University Mohammed V in Rabat, BP 6203, Rabat Instituts, Rabat 10100, Morocco
El Mostafa Karym
Laboratoire de Biochimie et Neurosciences, FST, Université Hassan I, BP 577, Settat 26000, Morocco
Nezha Saidi
Regional Office of Rabat, National Institute for Agricultural Research, P.O. Box 6570, Rabat Institutes, Rabat 10101, Morocco
Bouchra Meddah
Laboratoire de Pharmacologie et Toxicologie, équipe de Pharmacocinétique, Faculté de Médicine et Pharmacie, University Mohammed V in Rabat, BP 6203, Rabat Instituts, Rabat 10100, Morocco
Mourad Kharbach
Pharmaceutical and Toxicological Analysis Research Team, Laboratory of Pharmacology and Toxicology, Faculty of Medicine and Pharmacy, University Mohammed V, Rabat 10100, Morocco
Azlarab Masrar
Central Laboratory of Biochemistry, Ibn Sina Hospital, Rabat 10100, Morocco
Mounya Bouabdellah
Central Laboratory of Biochemistry, Ibn Sina Hospital, Rabat 10100, Morocco
Layachi Chabraoui
Central Laboratory of Biochemistry, Ibn Sina Hospital, Rabat 10100, Morocco
Khalid El Allali
Comparative Anatomy Unit-URAC-49, Hassan II Agronomy and Veterinary Institute, Rabat 10101, Morocco
Yahia Cherrah
Laboratoire de Pharmacologie et Toxicologie, équipe de Pharmacocinétique, Faculté de Médicine et Pharmacie, University Mohammed V in Rabat, BP 6203, Rabat Instituts, Rabat 10100, Morocco
My El Abbes Faouzi
Laboratoire de Pharmacologie et Toxicologie, équipe de Pharmacocinétique, Faculté de Médicine et Pharmacie, University Mohammed V in Rabat, BP 6203, Rabat Instituts, Rabat 10100, Morocco
Improvement of oat lines via introgression is an important process for food biochemical functionality. This work aims to evaluate the protective effect of phenolic compounds from hybrid Oat line (F11-5) and its parent (Amlal) on hyperglycemia-induced oxidative stress and to establish the possible mechanisms of antidiabetic activity by digestive enzyme inhibition. Eight phenolic acids were quantified in our samples including ferulic, p-hydroxybenzoic, caffeic, salicylic, syringic, sinapic, p-coumaric and chlorogenic acids. The Oat extract (2000 mg/kg) ameliorated the glucose tolerance, decreased Fasting Blood Glucose (FBG) and oxidative stress markers, including Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx), Glutathione (GSH) and Malondialdehyde (MDA) in rat liver and kidney. Furthermore, Metformin and Oat intake prevented anxiety, hypercholesterolemia and atherosclerosis in diabetic rats. In vivo anti-hyperglycemic effect of Oat extracts has been confirmed by their inhibitory activities on α-amylase (723.91 μg/mL and 1027.14 μg/mL) and α-glucosidase (1548.12 μg/mL & 1803.52 μg/mL) enzymes by mean of a mixed inhibition.
