Microbiome analysis of saliva from oral squamous cell carcinoma (OSCC) patients and tobacco abusers with potential biomarkers for oral cancer screening
Bolaji Fatai Oyeyemi,
Urvinder S. Kaur,
Amit Paramraj,
Chintamani,
Ravi Tandon,
Abhinav Kumar,
Neel Sarovar Bhavesh
Affiliations
Bolaji Fatai Oyeyemi
Transcription Regulation Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110067, India; Department of Science Technology, The Federal Polytechnic, P.M.B. 5351, Ado-Ekiti, Nigeria
Urvinder S. Kaur
Laboratory of AIDS Research and Immunology, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India
Amit Paramraj
Department of Surgery, Vardhman Mahavir Medical College (VMMC)-Safdarjung Hospital, New Delhi, 110058, India
Chintamani
Department of Surgery, Vardhman Mahavir Medical College (VMMC)-Safdarjung Hospital, New Delhi, 110058, India
Ravi Tandon
Laboratory of AIDS Research and Immunology, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India; Corresponding author. Laboratory of AIDS Research and Immunology, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India
Abhinav Kumar
Department of Surgery, Vardhman Mahavir Medical College (VMMC)-Safdarjung Hospital, New Delhi, 110058, India; Division of Trauma Surgery and Critical Care, Department of Surgical Disciplines, Jai Prakash Narayan Apex Trauma Center, All India Institute of Medical Sciences (AIIMS), New Delhi, 110029, India; Corresponding author. Division of Trauma Surgery and Critical Care, Department of Surgical Disciplines, Jai Prakash Narayan Apex Trauma Center, All India Institute of Medical Sciences (AIIMS), New Delhi, 110029, India
Neel Sarovar Bhavesh
Transcription Regulation Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110067, India; Corresponding author. Transcription Regulation Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi, 110067, India
Oral squamous cell carcinoma (OSCC) is the most common type of oral cancer and accounts for about 95% of all head and neck cancers with high mortality, usually at a late stage. Dysbiosis in the oral microbiome can lead to chronic inflammatory responses and may predispose to the development and progression of OSCC. Tobacco abuse plays an essential role in oral microbiome dysregulation and OSCC pathogenesis. We used 16S rRNA gene amplicon next-generation sequencing to examine microbial signatures unique to saliva from OSCC patients, tobacco abusers (TA) and controls (n = 10 for each group) to elucidate oral microbiome changes associated with tobacco abuse and OSCC. Overall, the oral microbiome compositions of class Betaproteobacteria and Epsilonproteobacteria, order Neisseriales, Burkholderiales and Campylobacterales, family Burkholderiaceae and Campylobacteraceae and genera Campylobacter and Leptotrichia revealed significant differences among OSCC patients, TA and control. Our preliminary pilot study not only serves as a basis for future studies with large sample size but also gives an indication of microbiome-based potential non-invasive biomarkers for early screening and monitoring of oral carcinogenesis transition due to tobacco abuse.
