Frontiers in Physiology (Aug 2020)
circAFF1 Aggravates Vascular Endothelial Cell Dysfunction Mediated by miR-516b/SAV1/YAP1 Axis
- Hong-guang Wang,
- Hong-guang Wang,
- Hong-guang Wang,
- Hong-guang Wang,
- Hua Yan,
- Hua Yan,
- Chen Wang,
- Chen Wang,
- Mi-mi Li,
- Xin-ze Lv,
- Hai-dong Wu,
- Zhan-hai Fang,
- Dong-li Mo,
- Dong-li Mo,
- Zhi-yuan Zhang,
- Bin Liang,
- Ke-guan Lai,
- Jing-yu Bao,
- Xue-jia Yang,
- Hong-juan Zhao,
- Shuang Chen,
- Yi-mu Fan,
- Yi-mu Fan,
- Yi-mu Fan,
- Xiao-guang Tong,
- Xiao-guang Tong,
- Xiao-guang Tong
Affiliations
- Hong-guang Wang
- College of Pharmacy, Nankai University, Tianjin, China
- Hong-guang Wang
- Department of Neurosurgery, Tianjin Huanhu Hospital, Tianjin, China
- Hong-guang Wang
- Department of Neurology, Tianjin Huanhu Hospital, Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases, Tianjin, China
- Hong-guang Wang
- Tianjin Institute, of Neurosurgery, Tianjin Huanhu Hospital, Tianjin, China
- Hua Yan
- Department of Neurosurgery, Tianjin Huanhu Hospital, Tianjin, China
- Hua Yan
- Department of Neurology, Tianjin Huanhu Hospital, Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases, Tianjin, China
- Chen Wang
- Department of Neurology, Tianjin Huanhu Hospital, Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases, Tianjin, China
- Chen Wang
- Tianjin Key Laboratory of Early Druggability Evaluation of Innovative Drugs, Tianjin International Joint Academy of Biomedicine, Tianjin, China
- Mi-mi Li
- Tianjin Institute, of Neurosurgery, Tianjin Huanhu Hospital, Tianjin, China
- Xin-ze Lv
- Drug Safety Evaluation Center, Tianjin International Joint Academy of Biomedicine, Tianjin, China
- Hai-dong Wu
- Tianjin Institute, of Neurosurgery, Tianjin Huanhu Hospital, Tianjin, China
- Zhan-hai Fang
- Department of Neurosurgery, People’s Hospital of Ningxia Hui Autonomous Region, Yinchuan, China
- Dong-li Mo
- Department of Neurology, Tianjin Huanhu Hospital, Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases, Tianjin, China
- Dong-li Mo
- Tianjin Key Laboratory of Early Druggability Evaluation of Innovative Drugs, Tianjin International Joint Academy of Biomedicine, Tianjin, China
- Zhi-yuan Zhang
- Tianjin Institute, of Neurosurgery, Tianjin Huanhu Hospital, Tianjin, China
- Bin Liang
- Tianjin Institute, of Neurosurgery, Tianjin Huanhu Hospital, Tianjin, China
- Ke-guan Lai
- Drug Safety Evaluation Center, Tianjin International Joint Academy of Biomedicine, Tianjin, China
- Jing-yu Bao
- Drug Safety Evaluation Center, Tianjin International Joint Academy of Biomedicine, Tianjin, China
- Xue-jia Yang
- Drug Safety Evaluation Center, Tianjin International Joint Academy of Biomedicine, Tianjin, China
- Hong-juan Zhao
- Department of Respiratory Medicine, Songjiang Sijing Hospital, Shanghai, China
- Shuang Chen
- Tianjin Key Laboratory of Early Druggability Evaluation of Innovative Drugs, Tianjin International Joint Academy of Biomedicine, Tianjin, China
- Yi-mu Fan
- Department of Neurosurgery, Tianjin Huanhu Hospital, Tianjin, China
- Yi-mu Fan
- Department of Neurology, Tianjin Huanhu Hospital, Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases, Tianjin, China
- Yi-mu Fan
- Tianjin Key Laboratory of Early Druggability Evaluation of Innovative Drugs, Tianjin International Joint Academy of Biomedicine, Tianjin, China
- Xiao-guang Tong
- Department of Neurosurgery, Tianjin Huanhu Hospital, Tianjin, China
- Xiao-guang Tong
- Department of Neurology, Tianjin Huanhu Hospital, Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases, Tianjin, China
- Xiao-guang Tong
- Tianjin Key Laboratory of Early Druggability Evaluation of Innovative Drugs, Tianjin International Joint Academy of Biomedicine, Tianjin, China
- DOI
- https://doi.org/10.3389/fphys.2020.00899
- Journal volume & issue
-
Vol. 11
Abstract
Pathological vascular endothelial damage caused by hypoxia is the basis of many vascular-related diseases. However, the role of circular RNA in hypoxic vascular injury is still poorly understood. Here, we found that hypoxia induced AFF1 circular RNA (circAFF1) can activate the SAV1/YAP1 and lead to the dysfunction of vascular endothelial cells. In HUV-EC-C and HBEC-5i cells, circAFF1 was upregulated under CoCl2 induced hypoxic conditions. The abnormal expression of circAFF1 inhibited the proliferation, tube formation, migration of vascular endothelial cells. The effect of circAFF1 is achieved by the adsorption of miR-516b to release SAV1, which in turn causes the phosphorylation of YAP1. Moreover, we found that the upregulation of circAFF1 in 235 Patients with subarachnoid hemorrhage. Taken together, we clarify the role of circAFF1/miR-516b/SAV1/YAP1 axis in vascular endothelial dysfunction and its potential early diagnostic value of disease caused by hypoxia injury in blood vessels.
Keywords
