Generation of urine iPS cell line from a patient with obsessive-compulsive disorder using a non-integrative method

Jaroslaw Sochacki; Sylvie Devalle; Marcelo Reis; Leonardo F. Fontenelle; Stevens Rehen

doi:10.1016/j.scr.2016.05.018

Stem Cell Research (Jul 2016)

Generation of urine iPS cell line from a patient with obsessive-compulsive disorder using a non-integrative method

Jaroslaw Sochacki,
Sylvie Devalle,
Marcelo Reis,
Leonardo F. Fontenelle,
Stevens Rehen

Affiliations

Jaroslaw Sochacki: D'Or Institute for Research and Education (IDOR), Rua Diniz Cordeiro, 30, Rio de Janeiro 222281, Brazil
Sylvie Devalle: D'Or Institute for Research and Education (IDOR), Rua Diniz Cordeiro, 30, Rio de Janeiro 222281, Brazil
Marcelo Reis: D'Or Institute for Research and Education (IDOR), Rua Diniz Cordeiro, 30, Rio de Janeiro 222281, Brazil
Leonardo F. Fontenelle: Obsessive, Compulsive, and Anxiety Spectrum Program, Institute of Psychiatry of the Federal University of Rio de Janeiro, Brazil
Stevens Rehen: D'Or Institute for Research and Education (IDOR), Rua Diniz Cordeiro, 30, Rio de Janeiro 222281, Brazil

DOI: https://doi.org/10.1016/j.scr.2016.05.018
Journal volume & issue: Vol. 17, no. 1
pp. 107 – 110

Abstract

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Urine sample was collected from a 29-year-old male patient with an early onset form of DSM-5 obsessive-compulsive disorder (OCD), comorbid body dysmorphic and excoriation (skin-picking) disorders, and a positive family history for OCD. Urine cell line was established and expanded for the reprogramming procedure. Induced pluripotent stem (iPS) cells were derived using the integration-free CytoTune®-iPS 2.0 Sendai Reprogramming Kit, which includes Sendai virus particles of the four Yamanaka factors Oct3/4, Sox2, Klf4 and c-Myc.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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