A gene cluster in Ginkgo biloba encodes unique multifunctional cytochrome P450s that initiate ginkgolide biosynthesis

Victor Forman; Dan Luo; Fernando Geu-Flores; René Lemcke; David R. Nelson; Sotirios C. Kampranis; Dan Staerk; Birger Lindberg Møller; Irini Pateraki

doi:10.1038/s41467-022-32879-9

Nature Communications (Sep 2022)

A gene cluster in Ginkgo biloba encodes unique multifunctional cytochrome P450s that initiate ginkgolide biosynthesis

Victor Forman,
Dan Luo,
Fernando Geu-Flores,
René Lemcke,
David R. Nelson,
Sotirios C. Kampranis,
Dan Staerk,
Birger Lindberg Møller,
Irini Pateraki

Affiliations

Victor Forman: Faculty of Science, Department of Plant and Environmental Sciences, Section for Plant Biochemistry, University of Copenhagen
Dan Luo: Faculty of Science, Department of Plant and Environmental Sciences, Section for Plant Biochemistry, University of Copenhagen
Fernando Geu-Flores: Faculty of Science, Department of Plant and Environmental Sciences, Section for Plant Biochemistry, University of Copenhagen
René Lemcke: Faculty of Health and Medical Sciences, Department of Neuroscience, University of Copenhagen
David R. Nelson: Faculty of Microbiology, Immunology and Biochemistry, The University of Tennessee Health Science Center, University of Tennessee
Sotirios C. Kampranis: Faculty of Science, Department of Plant and Environmental Sciences, Section for Plant Biochemistry, University of Copenhagen
Dan Staerk: Faculty of Health and Medical Sciences, Department of Drug Design and Pharmacology, University of Copenhagen
Birger Lindberg Møller: Faculty of Science, Department of Plant and Environmental Sciences, Section for Plant Biochemistry, University of Copenhagen
Irini Pateraki: Faculty of Science, Department of Plant and Environmental Sciences, Section for Plant Biochemistry, University of Copenhagen

DOI: https://doi.org/10.1038/s41467-022-32879-9
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 14

Abstract

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Although ginkgo terpenoids have been studied extensively for their pharmaceutical properties, knowledge on their biosynthesis remains limited. Here, the authors identify five multifunctional cytochrome P450s that catalyze the generation of the tert-butyl group and one of the lactone rings towards the biosynthesis of ginkgolides.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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