Treatment of Inactive Ovaries of Holstein Dairy Cows by Epidural Injection of GnRH Analogue (Receptal) and Its Impact on the Reproductive Hormones, Oxidant/Antioxidant Profile and Micro and Macro-Elements Profile
Yahia A. Amin,
Alaa Eldin Z. Mahmoud,
Rana A. Ali,
Samer S. Fouad,
Obeid Shanab,
Rawia M. Ibrahim,
Foad Farrag,
Mustafa Shukry,
Samah F. Ibrahim,
Liana Fericean,
Ragab Hassan Mohamed
Affiliations
Yahia A. Amin
Department of Theriogenology, Faculty of Veterinary Medicine, Aswan University, Aswan 81528, Egypt
Alaa Eldin Z. Mahmoud
Department of Theriogenology, Faculty of Veterinary Medicine, Sohag University, Sohag 82524, Egypt
Rana A. Ali
Department of Zoology, Faculty of Science, South Valley University, Qena 83523, Egypt
Samer S. Fouad
Clinical Pathology of Veterinary Medicine, Qena University Hospital, South Valley University, Qena 83523, Egypt
Obeid Shanab
Department of Biochemistry, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt
Rawia M. Ibrahim
Division of Clinical Laboratory Diagnosis, Animal Medicine Department, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt
Foad Farrag
Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt
This study was designed to evaluate a new therapeutic approach for inactive ovaries based on the epidural administration of a GnRH agonist (Receptal) and an investigation of the impact of this treatment on the hormonal, oxidant/antioxidant and micro- and macro-element profiles. Sixty cows with postpartum anestrus were divided into two groups: the first group (group Repid, n = 30) was administered an epidural injection of Receptal, while the second group (group Cepid, n = 30) received saline and was considered the control group. Evaluation of hormonal (progesterone, FSH, LH, testosterone, and cortisol), oxidant/antioxidant (MDA, SOD, GPx and TAC) as well as micro- and macroelement (calcium, phosphorus, manganese and magnesium) profiles was done in serum. The results showed that the epidural injection of Receptal has the potential to induce estrus response and conception incidence in treated cows. Compared to the control group, progesterone, FSH, and LH concentrations were significantly increased in the treated group, whereas testosterone and cortisol decreased (p p < 0.05) following receptal treatment, but differences in other minerals were not detected. This research suggests a novel, effective method of treating inactive ovaries with epidural infusion of a GnRH agonist.
