LncRNA GClnc1 promotes osteosarcoma progression by stabilizing NONO and blocking FBXW7-mediated ubiquitination
Jiongfeng Zhang,
Xiaohui Luo,
Chong Guo,
Zhengzai Dai,
Xiaofeng Tang,
Feifei Zhang,
Quanhui Jiao,
Shifan Lin,
Le Zou,
Zhiping Zhang,
Xiao-Bin Lv
Affiliations
Jiongfeng Zhang
Jiangxi Key Laboratory of Oncology, The Central Lab of The First Hospital of Nanchang, The Third Affiliated Hospital, Jiangxi Medical College, Nanchang University
Xiaohui Luo
Jiangxi Key Laboratory of Oncology, The Central Lab of The First Hospital of Nanchang, The Third Affiliated Hospital, Jiangxi Medical College, Nanchang University
Chong Guo
Jiangxi Key Laboratory of Oncology, The Central Lab of The First Hospital of Nanchang, The Third Affiliated Hospital, Jiangxi Medical College, Nanchang University
Zhengzai Dai
Jiangxi Key Laboratory of Oncology, The Central Lab of The First Hospital of Nanchang, The Third Affiliated Hospital, Jiangxi Medical College, Nanchang University
Xiaofeng Tang
Department of Orthopedics, Nanchang Key Laboratory of Orthopaedics, The First Hospital of Nanchang, The Third Affiliated Hospital, Jiangxi Medical College, Nanchang University
Feifei Zhang
Department of Orthopedics, Nanchang Key Laboratory of Orthopaedics, The First Hospital of Nanchang, The Third Affiliated Hospital, Jiangxi Medical College, Nanchang University
Quanhui Jiao
College of Pharmacy, Jiangxi University of Chinese Medicine
Shifan Lin
Jiangxi Key Laboratory of Oncology, The Central Lab of The First Hospital of Nanchang, The Third Affiliated Hospital, Jiangxi Medical College, Nanchang University
Le Zou
Jiangxi Key Laboratory of Oncology, The Central Lab of The First Hospital of Nanchang, The Third Affiliated Hospital, Jiangxi Medical College, Nanchang University
Zhiping Zhang
Jiangxi Key Laboratory of Oncology, The Central Lab of The First Hospital of Nanchang, The Third Affiliated Hospital, Jiangxi Medical College, Nanchang University
Xiao-Bin Lv
Department of Orthopedics, Nanchang Key Laboratory of Orthopaedics, The First Hospital of Nanchang, The Third Affiliated Hospital, Jiangxi Medical College, Nanchang University
Abstract Background Long non-coding RNA (lncRNA) plays a vital role in the occurrence and development of varieties of tumors. Previous studies have shown that lncRNA GClnc1 is highly expressed in osteosarcoma (OS). However, the mechanism of lncRNA GClnc1 in osteosarcoma has not been fully elucidated. In this study, we investigated the biological roles of lncRNA GClnc1 in osteosarcoma and unveiled its underlying mechanisms. Methods The expression of lncRNA GClnc1 in OS cells was detected by real-time quantitative PCR (qRT-PCR). The functional roles of lncRNA GClnc1 were examined by CCK8, trans-well, scratch wound healing assay, colony formation, and apoptosis assays in osteosarcoma cells upon silencing or overexpressing GClnc1. Western blot analysis, qRT-PCR, and RNA co-immunoprecipitation (RIP) assays were used to detect the interaction between lncRNA GClnc1 and NONO. Results The expression of lncRNA GClnc1 was up-regulated in osteosarcoma cell lines. Knockdown of lncRNA GClnc1 suppressed the cell growth, migration, and invasion of OS cells, whereas the over-expression of GClnc1 improved the proliferation, migration, and invasion of OS cells. Mechanistically, we identified that lncRNA GClnc1 regulates the stability of NONO by blocking FBXW7-mediated ubiquitination degradation. Additionally, overexpression of NONO can reverse GClnc1 silencing exerted suppression of the cell proliferation, migration, and invasion, and vice versa. Conclusions Our study elucidated that lncRNA GClnc1 participates in the progression of OS by regulating the NONO signal pathway. Targeting GClnc1 provides a potential target for future clinical treatment of OS.
