Observation on Hemicriconemoides brachyurus (Loos, 1949) Chitwood & Birchfield, 1957 associated with grass in South Africa

Abstract

Hemicriconemoides brachyurus was identified morphologically, using body annuli number (103105), stylet length (49-52 μm), tail conical with small rounded lobe, spermatheca with round sperm, and VL/VB (1.3). Molecular analysis was also undertaken, based on the 18S rDNA region, and the obtained DNA sequence data confirmed the present population from South Africa as Hemicriconemoides. Phylogenetic analysis using the Bayesian inference method places this population close to H. fujianensis from China (MH444620-21; MH444626-28). To the best of our knowledge, this is the first report of the 18S rDNA sequence for H. brachyurus.

Keywords

• south africa
• grass
• hemicriconemoides
• morphology
• 18s rdna