Bolʹ, Sustavy, Pozvonočnik (Mar 2020)
Reference intervals of serum carboxy-terminal telopeptide of type I collagen in the Ukrainian residents of various ages and sexes
Abstract
Background. The purpose of the study is to determine the reference intervals of carboxy-terminal telopeptide of type I collagen (β-CTX) in the blood serum of healthy males and females of various ages, residents of Ukraine. Materials and methods. The study included 4754 individuals aged 20–89 years (4123 women (mean age 59.6 ± 13.6) and 631 men (mean age 49.5 ± 16.3)). All participants were divided into 7 groups by the age decades (from 20 to 89 years), and three groups of 40–49, 50–59 and 60–69 year-olds were additionally divided into the subgroups by five years. Furthermore, the patients were divided according to the gerontological classification of age into 4 groups: young — 20–44 years, middle-age — 45–59 years, elderly — 60–74 years, and old — 75–89 years. The blood serum β-CTX concentration was determined by ECLIA electrochemiluminescence immunoassay on the cobas e 411 analyzer. Results. We have detected significant effect of age on serum β-CTX variability: a significant increase in β-CTX levels in women (F = 15.48, p < 0.00001), and a decrease in men (F = 11.73, p < 0.00001) with age. The significantly lower levels of β-CTX were found in the women aged 20–29 (F = 35.68, p < 0.00001) and 30–39 years (F = 19.47, p = 0.00001) compared to the men of similar ages. However, the women of 65–69 years (F = 10.50, p = 0.001) had a significantly higher serum β-CTX levels compared to men of the same age group. Conclusions. The serum β-CTX levels have been associated with age: in men they are decreasing significantly with age, in women — significantly increasing. In the young men aged 20–44 years and women aged 65–69 years, the β-CTX concentration was significantly higher compared with that of the opposite sex subjects of a similar age. The findings on β-CTX serum levels in the practically healthy men and women of various ages, who are residing in this country, may be used as the reference intervals for the Ukrainian population.
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