Effects of <i>Bacillus amyloliquefaciens</i> XJ-BV2007 on Growth of <i>Alternaria alternata</i> and Production of Tenuazonic Acid
Qinlan Jia,
Yingying Fan,
Shuaishuai Duan,
Qiaomei Qin,
Yu Ding,
Min Yang,
Yan Wang,
Fengjuan Liu,
Cheng Wang
Affiliations
Qinlan Jia
College of Life Science and Technology, Xinjiang University, Urumqi 830049, China
Yingying Fan
Key Laboratory of Agro-products Quality and Safety of Xinjiang, Laboratory of Quality and Safety Risk Assessment for Agri-products (Urumqi), Institute of Quality Standards & Testing Technology for Agri-products, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
Shuaishuai Duan
College of Life Science and Technology, Xinjiang University, Urumqi 830049, China
Qiaomei Qin
College of Life Science and Technology, Xinjiang University, Urumqi 830049, China
Yu Ding
College of Biology and Geography Sciences, Yili Normal University, Yining 835000, China
Min Yang
College of Biology and Geography Sciences, Yili Normal University, Yining 835000, China
Yan Wang
Key Laboratory of Agro-products Quality and Safety of Xinjiang, Laboratory of Quality and Safety Risk Assessment for Agri-products (Urumqi), Institute of Quality Standards & Testing Technology for Agri-products, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
Fengjuan Liu
College of Life Science and Technology, Xinjiang University, Urumqi 830049, China
Cheng Wang
College of Life Science and Technology, Xinjiang University, Urumqi 830049, China
Large amounts of processing tomato are grown in Xinjiang, China. Tomato black spot disease, caused by Alternaria spp., and the produced alternaria toxins in tomato products are posing risks to human health. In this study, we isolated a rhizospheric bacterium, XJ-BV2007, from tomato (Solanum lycopersicum) fields, which we identified as Bacillus amyloliquefaciens. We found that this bacterium has a strong antagonistic effect against Alternaria alternata and reduces the accumulation of alternaria toxins in tomatoes. According to the antifungal activity of the bacteria-free filtrate, we revealed that B. amyloliquefaciens XJ-BV2007 suppresses A. alternata by the production of antifungal metabolites. Combining semi-preparative high-performance liquid chromatography, we employed UPLC-QTOF-MS analysis and the Oxford cup experiment to find that fengycin plays an important role in inhibiting A. alternata. This paper firstly reported that B. amyloliquefaciens efficiently controls tomato black spot disease and mycotoxins caused by A. alternata. B. amyloliquefaciens XJ-BV2007 may provide an alternative biocontrol strain for the prevention of tomato black spot disease.
