EXPRESS-DIAGNOSTIC OF RUBELLA INFECTION BY THE IMMUNOFLUORESCENT ASSAY USING MONOCLONAL ANTIBODIES TO PROTEIN E1 OF RUBELLA VIRUS

Abstract

Abstract. The high rubella incidence rate in the Russian Federation and the low registration level of the rubella congenital syndrome which is not reflect the epidemic situation requiring optimization of the rubella diagnostics. In the present study the possibility of early detection of rubella virus in case of inoculation of sensitive cell culture by the nasopharyngeal washouts with following detection of rubella antigen with highly specific monoclonal antibodies to the basic structural protein E1 of rubella virus using direct immunofluorescent method have been shown. Using of the rapid cell cultural method allows providing etiological diagnosis of high specificity within 5 hours after inoculation of virus to the sensitive cells Vero E6 or BHK-21-F.

Keywords

• rapid culture method
• sensitive sells
• rubella virus
• monoclonal antibodies
• immune-fluorescent assay