مجله بیوتکنولوژی کشاورزی (May 2011)

The Effect of Different Light Intensities on Callogenesis and Calli Pigments Content of Shoot and Floral Explants of Rosa damascena Mill. and Rosa miniature

  • Somayeh Abdirad,
  • Farkhondeh Reza nejad,
  • KHosroo Manoochehri

DOI
https://doi.org/10.22103/jab.2012.460
Journal volume & issue
Vol. 3, no. 1
pp. 43 – 65

Abstract

Read online

This study was conducted to compare the ability of callus production between shoot (leaf, petiole and stem) and flower (petal, pistil and anther) organs, as well as chlorophyll and anthocyanin accumulation in calli obtained from these explants in dark and light in Rosa miniature and Rosa damascena. Miniature rose and damask rose have become increasingly popular and economically important in recent years. In vitro regeneration method has a great commercial value in the rose propagation industry. The sterilized explants were cultured in modified MS medium supplemented by various concentrations of 2, 4-D (1, 2, 3, and 4 mg.l-1) and BAP (0.1, 0.5, 1 and 1.5 mg.l-1). The samples were kept under 1000 and 2000 lux white light intensities and dark. Combinations of 4 mg l-1 2, 4-D and 1.5 mg l-1 BAP in Rosa damascena were the most suitable treatments for callus production whereas various explants of Rosa miniature had different response to different growth regulator combinations. In the dark, callus initiation was quicker compared to the light. In addition, callus production of shoot explants were begun faster than floral ones. Callus initiation of floral explants from Rosa miniature occurred in 2th week after culturing, whereas in Rosa damascena observed in 3th week. The growth rate of calli from all explants (except anther) of Rosa miniature was more than Rosa damascena. The dark grown calli contained very low chlorophyll and anthocyanin concentrations whereas these contents increased remarkably at 1000 and 2000 lux (especially 2000 lux) light intensity. Leaf calli in Rosa damascena and stem calli in Rosa miniature produced maximum values of chlorophyll pigments whereas in both, anther and petiole calli had minimum amounts. The calli obtained of petal and pistil explants accumulated high concentration of anthocyanin pigments whereas the lowest values recorded from anther and petiole calli in Rosa miniature and petiole calli in Rosa damascena.

Keywords