Engineering of a bona fide light-operated calcium channel

Lian He; Liuqing Wang; Hongxiang Zeng; Peng Tan; Guolin Ma; Sisi Zheng; Yaxin Li; Lin Sun; Fei Dou; Stefan Siwko; Yun Huang; Youjun Wang; Yubin Zhou

doi:10.1038/s41467-020-20425-4

Nature Communications (Jan 2021)

Affiliations

Lian He: Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University
Liuqing Wang: Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University
Hongxiang Zeng: Center for Epigenetics and Disease Prevention, Institute of Biosciences and Technology, Texas A&M University
Peng Tan: Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University
Guolin Ma: Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University
Sisi Zheng: Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University
Yaxin Li: Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University
Lin Sun: Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University
Fei Dou: Beijing Key Laboratory of Genetic Engineering Drugs and Biotechnology, College of Life Sciences, Beijing Normal University
Stefan Siwko: Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University
Yun Huang: Center for Epigenetics and Disease Prevention, Institute of Biosciences and Technology, Texas A&M University
Youjun Wang: Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University
Yubin Zhou: Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University

DOI: https://doi.org/10.1038/s41467-020-20425-4
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 11

Abstract

Read online

Existing optogenetic methods to induce calcium mobilisation lack selectivity and specificity. Here, the authors design and engineer a single-component light-operated calcium channel to provide optical control over calcium signals and calcium-dependent physiological responses: LOCa.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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