Sodium Hydrosulfide Treatment During Porcine Kidney Ex Vivo Perfusion and Transplantation

Thomas Agius, MS; Julien Songeon, MS; Arnaud Lyon, MD; Justine Longchamp, MD; Raphael Ruttimann, BS; Florent Allagnat, PhD; Sébastien Déglise, MD; Jean-Marc Corpataux, MD; Déla Golshayan, MD, PhD; Léo Buhler, MD; Raphael Meier, MD, PhD; Heidi Yeh, MD; James F. Markmann, MD, PhD; Korkut Uygun, PhD; Christian Toso, MD, PhD; Antoine Klauser, PhD; Francois Lazeyras, PhD; Alban Longchamp, MD, PhD

doi:10.1097/TXD.0000000000001508

Transplantation Direct (Nov 2023)

Sodium Hydrosulfide Treatment During Porcine Kidney Ex Vivo Perfusion and Transplantation

Thomas Agius, MS,
Julien Songeon, MS,
Arnaud Lyon, MD,
Justine Longchamp, MD,
Raphael Ruttimann, BS,
Florent Allagnat, PhD,
Sébastien Déglise, MD,
Jean-Marc Corpataux, MD,
Déla Golshayan, MD, PhD,
Léo Buhler, MD,
Raphael Meier, MD, PhD,
Heidi Yeh, MD,
James F. Markmann, MD, PhD,
Korkut Uygun, PhD,
Christian Toso, MD, PhD,
Antoine Klauser, PhD,
Francois Lazeyras, PhD,
Alban Longchamp, MD, PhD

Affiliations

Thomas Agius, MS: 1 Department of Vascular Surgery, Lausanne University Hospital, Lausanne, Switzerland.
Julien Songeon, MS: 4 Department of Radiology and Medical Informatics, University of Geneva, Geneva, Switzerland.
Arnaud Lyon, MD: 1 Department of Vascular Surgery, Lausanne University Hospital, Lausanne, Switzerland.
Justine Longchamp, MD: 1 Department of Vascular Surgery, Lausanne University Hospital, Lausanne, Switzerland.
Raphael Ruttimann, BS: 6 Visceral and Transplant Surgery, Department of Surgery, Geneva University Hospitals and Medical School, Geneva, Switzerland.
Florent Allagnat, PhD: 1 Department of Vascular Surgery, Lausanne University Hospital, Lausanne, Switzerland.
Sébastien Déglise, MD: 1 Department of Vascular Surgery, Lausanne University Hospital, Lausanne, Switzerland.
Jean-Marc Corpataux, MD: 1 Department of Vascular Surgery, Lausanne University Hospital, Lausanne, Switzerland.
Déla Golshayan, MD, PhD: 5 Department of Medicine, Transplantation Centre, Lausanne University Hospital, Lausanne, Switzerland.
Léo Buhler, MD: 7 Section of Medicine, Faculty of Science and Medicine, University of Fribourg, Switzerland.
Raphael Meier, MD, PhD: 8 Department of Surgery, University of Maryland School of Medicine, Baltimore, MD.
Heidi Yeh, MD: 2 Department of Surgery, Transplant Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA.
James F. Markmann, MD, PhD: 2 Department of Surgery, Transplant Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA.
Korkut Uygun, PhD: 3 Department of Surgery, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA.
Christian Toso, MD, PhD: 6 Visceral and Transplant Surgery, Department of Surgery, Geneva University Hospitals and Medical School, Geneva, Switzerland.
Antoine Klauser, PhD: 4 Department of Radiology and Medical Informatics, University of Geneva, Geneva, Switzerland.
Francois Lazeyras, PhD: 4 Department of Radiology and Medical Informatics, University of Geneva, Geneva, Switzerland.
Alban Longchamp, MD, PhD: 1 Department of Vascular Surgery, Lausanne University Hospital, Lausanne, Switzerland.

DOI: https://doi.org/10.1097/TXD.0000000000001508
Journal volume & issue: Vol. 9, no. 11
p. e1508

Abstract

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Background. In rodents, hydrogen sulfide (H2S) reduces ischemia-reperfusion injury and improves renal graft function after transplantation. Here, we hypothesized that the benefits of H2S are conserved in pigs, a more clinically relevant model. Methods. Adult porcine kidneys retrieved immediately or after 60 min of warm ischemia (WI) were exposed to 100 µM sodium hydrosulfide (NaHS) (1) during the hypothermic ex vivo perfusion only, (2) during WI only, and (3) during both WI and ex vivo perfusion. Kidney perfusion was evaluated with dynamic contrast-enhanced MRI. MRI spectroscopy was further employed to assess energy metabolites including ATP. Renal biopsies were collected at various time points for histopathological analysis. Results. Perfusion for 4 h pig kidneys with Belzer MPS UW + NaHS resulted in similar renal perfusion and ATP levels than perfusion with UW alone. Similarly, no difference was observed when NaHS was administered in the renal artery before ischemia. After autotransplantation, no improvement in histologic lesions or cortical/medullary kidney perfusion was observed upon H2S administration. In addition, AMP and ATP levels were identical in both groups. Conclusions. In conclusion, treatment of porcine kidney grafts using NaHS did not result in a significant reduction of ischemia-reperfusion injury or improvement of kidney metabolism. Future studies will need to define the benefits of H2S in human, possibly using other molecules as H2S donors.

Published in Transplantation Direct

ISSN: 2373-8731 (Online)
Publisher: Wolters Kluwer
Country of publisher: United States
LCC subjects: Medicine: Surgery
Website: http://journals.lww.com/transplantationdirect/Pages/default.aspx

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