The Revisited Genome of <i>Bacillus subtilis</i> Bacteriophage SPP1
Lia M. Godinho,
Mehdi El Sadek Fadel,
Céline Monniot,
Lina Jakutyte,
Isabelle Auzat,
Audrey Labarde,
Karima Djacem,
Leonor Oliveira,
Rut Carballido-Lopez,
Silvia Ayora,
Paulo Tavares
Affiliations
Lia M. Godinho
Institut de Biologie Intégrative de la Cellule (I2BC), French Alternative Energies and Atomic Energy Commission (CEA), Centre National de la Recherche Scientifique (CNRS), Univ Paris-Sud, Université Paris-Saclay, 91190 Gif-sur-Yvette, France
Mehdi El Sadek Fadel
Institut de Biologie Intégrative de la Cellule (I2BC), French Alternative Energies and Atomic Energy Commission (CEA), Centre National de la Recherche Scientifique (CNRS), Univ Paris-Sud, Université Paris-Saclay, 91190 Gif-sur-Yvette, France
Céline Monniot
MICALIS, Institut National de la Recherche Agronomique (INRA), AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France
Lina Jakutyte
Unité de Virologie Moléculaire et Structurale (VMS), CNRS, 91198 Gif-sur-Yvette, France
Isabelle Auzat
Institut de Biologie Intégrative de la Cellule (I2BC), French Alternative Energies and Atomic Energy Commission (CEA), Centre National de la Recherche Scientifique (CNRS), Univ Paris-Sud, Université Paris-Saclay, 91190 Gif-sur-Yvette, France
Audrey Labarde
Institut de Biologie Intégrative de la Cellule (I2BC), French Alternative Energies and Atomic Energy Commission (CEA), Centre National de la Recherche Scientifique (CNRS), Univ Paris-Sud, Université Paris-Saclay, 91190 Gif-sur-Yvette, France
Karima Djacem
Institut de Biologie Intégrative de la Cellule (I2BC), French Alternative Energies and Atomic Energy Commission (CEA), Centre National de la Recherche Scientifique (CNRS), Univ Paris-Sud, Université Paris-Saclay, 91190 Gif-sur-Yvette, France
Leonor Oliveira
Institut de Biologie Intégrative de la Cellule (I2BC), French Alternative Energies and Atomic Energy Commission (CEA), Centre National de la Recherche Scientifique (CNRS), Univ Paris-Sud, Université Paris-Saclay, 91190 Gif-sur-Yvette, France
Rut Carballido-Lopez
MICALIS, Institut National de la Recherche Agronomique (INRA), AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France
Silvia Ayora
Centro Nacional de Biotecnología (CNB-CSIC), 28049 Madrid, Spain
Paulo Tavares
Institut de Biologie Intégrative de la Cellule (I2BC), French Alternative Energies and Atomic Energy Commission (CEA), Centre National de la Recherche Scientifique (CNRS), Univ Paris-Sud, Université Paris-Saclay, 91190 Gif-sur-Yvette, France
Bacillus subtilis bacteriophage SPP1 is a lytic siphovirus first described 50 years ago. Its complete DNA sequence was reported in 1997. Here we present an updated annotation of the 44,016 bp SPP1 genome and its correlation to different steps of the viral multiplication process. Five early polycistronic transcriptional units encode phage DNA replication proteins and lysis functions together with less characterized, mostly non-essential, functions. Late transcription drives synthesis of proteins necessary for SPP1 viral particles assembly and for cell lysis, together with a short set of proteins of unknown function. The extensive genetic, biochemical and structural biology studies on the molecular mechanisms of SPP1 DNA replication and phage particle assembly rendered it a model system for tailed phages research. We propose SPP1 as the reference species for a new SPP1-like viruses genus of the Siphoviridae family.
