Tailoring the evolution of BL21(DE3) uncovers a key role for RNA stability in gene expression toxicity

Abstract

Heyde and Nørholm investigate the role of RNAse E in the toxicity mediated by overexpression of heterologous proteins in derivatives of the BL21(DE3) strain of E. coli. The authors report an unexpected connection between global RNA metabolism and the efficiency of heterologous protein production in E. coli.