International Journal of General Medicine (Jul 2022)
Effect of Tensile Frequency on the Osteogenic Differentiation of Periodontal Ligament Stem Cells
Abstract
Wenfang Wang,1,* Meijuan Wang,2,* Xiaomei Guo,3– 5 Yunshan Zhao,1 Madiha Mohammed Saleh Ahmed,1 Hong Qi,3– 5 Xi Chen1 1Department of Stomatology, First Affiliated Hospital, College of Medicine, Xi’an Jiaotong University, Xi’an, 710000, People’s Republic of China; 2Anesthesiology Department, Second Affiliated Hospital, College of Medicine, Xi’an Jiaotong University, Xi’an, 710000, People’s Republic of China; 3Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong University, Xi’an, 710000, People’s Republic of China; 4Laboratory Center of Stomatology, College of Stomatology, Xi’an Jiaotong University, Xi’an, 710000, People’s Republic of China; 5Department of Pathology, College of Stomatology, Xi’an Jiaotong University, Xi’an, 710000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hong Qi, Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong University, 98 Xiwu Road, Xi’an, 710000, People’s Republic of China, Tel +86-18161839153, Email [email protected] Xi Chen, Department of Stomatology, First Affiliated Hospital, College of Medicine, Xi’an Jiaotong University, 227 West Yanta Road, Xi’an, 710000, People’s Republic of China, Tel +86-13038598996, Email [email protected]: The role of periodontal ligament stem cells (PDLSCs) in mediating osteogenesis involved in orthodontic tooth movement (OTM) is well established. However, various relevant in vitro studies vary in the frequency of tension. The effect of tensile frequency on the mechanotransduction of PDLSCs is not clear. The current study aimed to determine the effect of different tensile frequencies on the osteogenic differentiation of PDLSCs and to identify important mechano-sensitivity genes.Methods: Human PDLSCs were isolated, identified, and subjected to cyclic equibiaxial tensile strain of 12% at different frequencies of 0.1 Hz, 0.5 Hz, 0.7 Hz, or static cultures. Osteogenic differentiation of PDLSCs was assessed by using Western blotting. High-throughput sequencing was used to identify differential mRNA expression. Short time-series expression miner (STEM) was utilized to describe the frequency patterns of the mRNAs. The functions and enriched pathways were identified, and the hub genes were identified and validated.Results: We found that the osteoblastic differentiation capacity of PDLSCs increased with tensile frequency in the range of 0.1– 0.7 Hz. Eight frequency-tendency gene expression profiles were identified to be statistically significant. Tensile frequency-specific expressed genes, such as SALL1 and EYA1, which decreased with the increase in tensile frequency, were found.Conclusion: The osteoblastic differentiation of PDLSCs under mechanical tensile force is frequency dependent. EYA1 and SALL1 were identified as potential important tensile frequency-sensitive genes, which may contribute to the cyclic tension-induced osteogenic differentiation of PDLSCs in a frequency-dependent manner.Keywords: cyclic tension, tensile frequency, osteogenesis, osteoblastic differentiation, periodontal ligament stem cells
