Frontiers in Molecular Neuroscience (Jan 2024)

Methods to extract and analyze fluid from human pluripotent stem cell-derived choroid plexus organoids

  • Leon H. Chew,
  • Eloi Mercier,
  • Jason C. Rogalski,
  • Sara Pippard,
  • Erin Knock

DOI
https://doi.org/10.3389/fnmol.2023.1243499
Journal volume & issue
Vol. 16

Abstract

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The choroid plexus (ChP) is a highly vascularized tissue lining the ventricular space of the brain. The ChP generates cerebrospinal fluid (CSF) and forms a protective barrier in the central nervous system (CNS). Recently, a three-dimensional human pluripotent stem cell (hPSC)-derived ChP organoid model has been developed. This model generates cystic structures that are filled with a fluid resembling CSF and are surrounded by an epithelial layer expressing ependymal choroid plexus-specific markers. Here we describe a method to generate these choroid plexus organoids using a commercial kit and methods to extract the CSF-like fluid for use in downstream analysis.

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