The Quantitative Trait Loci Mapping of Rice Plant and the Components of Its Extract Confirmed the Anti-Inflammatory and Platelet Aggregation Effects In Vitro and In Vivo
Jae-Ryoung Park,
Rahmatullah Jan,
Seul-Gi Park,
Tri Handoyo,
Gang-Seob Lee,
Sopheap Yun,
Yoon-Hee Jang,
Xiao-Xuan Du,
Taeho Lee,
Yong-Sham Kwon,
Doh Hoon Kim,
Young-Mi Seok,
Jong-Sup Bae,
Kyung-Min Kim
Affiliations
Jae-Ryoung Park
Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture & Life Science, Kyungpook National University, 80 Dahak-ro, Buk-gu, Daegu 41566, Korea
Rahmatullah Jan
Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture & Life Science, Kyungpook National University, 80 Dahak-ro, Buk-gu, Daegu 41566, Korea
Seul-Gi Park
National Institute of Crop Science, Rural Development Administration, Jeonju 54874, Korea
Tri Handoyo
Department of Agronomy, Faculty of Agriculture, Jember University, Jl. Kalimantan 37, Jember 68121, Indonesia
Gang-Seob Lee
Biosafety Division, National Academy of Agricultural Science, Rural Development Administration, Jeonju 54874, Korea
Sopheap Yun
Graduate School of Science, Royal University of Phnom Penh, Sangkat Teuk Laak 1, Russian Federation Boulevard, Toul Kork, Phnom Penh 12101, Cambodia
Yoon-Hee Jang
Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture & Life Science, Kyungpook National University, 80 Dahak-ro, Buk-gu, Daegu 41566, Korea
Xiao-Xuan Du
Costal Agriculture Research Institute, Kyungpook National University, 80 Dahak-ro, Buk-gu, Daegu 41566, Korea
Taeho Lee
College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, 80 Dahak-ro, Buk-gu, Daegu 41566, Korea
Yong-Sham Kwon
Department of Genetic Engineering, College of Natural Resources and Life Science, Dong-A University, Busan 49315, Korea
Doh Hoon Kim
Department of Genetic Engineering, College of Natural Resources and Life Science, Dong-A University, Busan 49315, Korea
Young-Mi Seok
Department of Korean Medicine Development, National Institute for Korean Medicine Development, 94, Hwarang-ro, Gyeongsan-si 38540, Gyeongsangbuk-do, Korea
Jong-Sup Bae
College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, 80 Dahak-ro, Buk-gu, Daegu 41566, Korea
Kyung-Min Kim
Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture & Life Science, Kyungpook National University, 80 Dahak-ro, Buk-gu, Daegu 41566, Korea
Unpredictable climate change might cause serious lack of food in the world. Therefore, in the present world, it is urgent to prepare countermeasures to solve problems in terms of human survival. In this research, quantitative trait loci (QTLs) were analyzed when rice attacked by white backed planthopper (WBPH) were analyzed using 120 Cheongcheong/Nagdong double haploid lines. Moreover, from the detected QTLs, WBPH resistance-related genes were screened in large candidate genes. Among them, OsCM, a major gene in the synthesis of Cochlioquinone-9 (cq-9), was screened. OsCM has high homology with the sequence of chorismate mutase, and exists in various functional and structural forms in plants that produce aromatic amino acids. It also induces resistance to biotic stress through the synthesis of secondary metabolites in plants. The WBPH resistance was improved in rice overexpressed through map-based cloning of the WBPH resistance-related gene OsCM, which was finally detected by QTL mapping. In addition, cq-9 increased the survival rate of caecal ligation puncture (CLP)-surgery mice by 60%. Moreover, the aorta of rat treated with cq-9 was effective in vasodilation response and significantly reduced the aggregation of rat platelets induced by collagen treatment. A cq-9, which is strongly associated with resistance to WBPH in rice, is also associated with positive effect of CLP surgery mice survival rate, vasodilation, and significantly reduced rat platelet aggregation induced by collagen treatment. Therefore, cq-9 presents research possibilities as a substance in a new paradigm that can act on both Plant-Insect in response to the present unpredictable future.
