Two-tiered enforcement of high-fidelity DNA ligation

Percy P. Tumbale; Thomas J. Jurkiw; Matthew J. Schellenberg; Amanda A. Riccio; Patrick J O’Brien; R. Scott Williams

doi:10.1038/s41467-019-13478-7

Nature Communications (Nov 2019)

Two-tiered enforcement of high-fidelity DNA ligation

Percy P. Tumbale,
Thomas J. Jurkiw,
Matthew J. Schellenberg,
Amanda A. Riccio,
Patrick J O’Brien,
R. Scott Williams

Affiliations

Percy P. Tumbale: Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, US National Institutes of Health, Department of Health and Human Services
Thomas J. Jurkiw: Biological Chemistry, University of Michigan
Matthew J. Schellenberg: Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, US National Institutes of Health, Department of Health and Human Services
Amanda A. Riccio: Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, US National Institutes of Health, Department of Health and Human Services
Patrick J O’Brien: Biological Chemistry, University of Michigan
R. Scott Williams: Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, US National Institutes of Health, Department of Health and Human Services

DOI: https://doi.org/10.1038/s41467-019-13478-7
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 13

Abstract

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DNA ligases catalyze the joining of DNA strands to complete DNA replication, recombination and repair transactions. Here the authors present X-ray structures and kinetic analyses of LIG1 complexes with undamaged and oxidatively damaged DNA that unveil determinants of LIG1 substrate recognition and enzymatic fidelity.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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