Sensors (Jul 2024)

Raman-Activated, Interactive Sorting of Isotope-Labeled Bacteria

  • Sepehr Razi,
  • Nicolae Tarcea,
  • Thomas Henkel,
  • Ramya Ravikumar,
  • Aikaterini Pistiki,
  • Annette Wagenhaus,
  • Sophie Girnus,
  • Martin Taubert,
  • Kirsten Küsel,
  • Petra Rösch,
  • Jürgen Popp

DOI
https://doi.org/10.3390/s24144503
Journal volume & issue
Vol. 24, no. 14
p. 4503

Abstract

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Due to its high spatial resolution, Raman microspectroscopy allows for the analysis of single microbial cells. Since Raman spectroscopy analyzes the whole cell content, this method is phenotypic and can therefore be used to evaluate cellular changes. In particular, labeling with stable isotopes (SIPs) enables the versatile use and observation of different metabolic states in microbes. Nevertheless, static measurements can only analyze the present situation and do not allow for further downstream evaluations. Therefore, a combination of Raman analysis and cell sorting is necessary to provide the possibility for further research on selected bacteria in a sample. Here, a new microfluidic approach for Raman-activated continuous-flow sorting of bacteria using an optical setup for image-based particle sorting with synchronous acquisition and analysis of Raman spectra for making the sorting decision is demonstrated, showing that active cells can be successfully sorted by means of this microfluidic chip.

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