Journal of Extracellular Vesicles (Feb 2024)
Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches
- Joshua A. Welsh,
- Deborah C. I. Goberdhan,
- Lorraine O'Driscoll,
- Edit I. Buzas,
- Cherie Blenkiron,
- Benedetta Bussolati,
- Houjian Cai,
- Dolores Di Vizio,
- Tom A. P. Driedonks,
- Uta Erdbrügger,
- Juan M. Falcon‐Perez,
- Qing‐Ling Fu,
- Andrew F. Hill,
- Metka Lenassi,
- Sai Kiang Lim,
- Mỹ G. Mahoney,
- Sujata Mohanty,
- Andreas Möller,
- Rienk Nieuwland,
- Takahiro Ochiya,
- Susmita Sahoo,
- Ana C. Torrecilhas,
- Lei Zheng,
- Andries Zijlstra,
- Sarah Abuelreich,
- Reem Bagabas,
- Paolo Bergese,
- Esther M. Bridges,
- Marco Brucale,
- Dylan Burger,
- Randy P. Carney,
- Emanuele Cocucci,
- Rossella Crescitelli,
- Edveena Hanser,
- Adrian L. Harris,
- Norman J. Haughey,
- An Hendrix,
- Alexander R. Ivanov,
- Tijana Jovanovic‐Talisman,
- Nicole A. Kruh‐Garcia,
- Vroniqa Ku'ulei‐Lyn Faustino,
- Diego Kyburz,
- Cecilia Lässer,
- Kathleen M. Lennon,
- Jan Lötvall,
- Adam L. Maddox,
- Elena S. Martens‐Uzunova,
- Rachel R. Mizenko,
- Lauren A. Newman,
- Andrea Ridolfi,
- Eva Rohde,
- Tatu Rojalin,
- Andrew Rowland,
- Andras Saftics,
- Ursula S. Sandau,
- Julie A. Saugstad,
- Faezeh Shekari,
- Simon Swift,
- Dmitry Ter‐Ovanesyan,
- Juan P. Tosar,
- Zivile Useckaite,
- Francesco Valle,
- Zoltan Varga,
- Edwin van derPol,
- Martijn J. C. vanHerwijnen,
- Marca H. M. Wauben,
- Ann M. Wehman,
- Sarah Williams,
- Andrea Zendrini,
- Alan J. Zimmerman,
- MISEV Consortium,
- Clotilde Théry,
- Kenneth W. Witwer
Affiliations
- Joshua A. Welsh
- Translational Nanobiology Section, Laboratory of Pathology National Cancer Institute, National Institutes of Health Bethesda Maryland USA
- Deborah C. I. Goberdhan
- Nuffield Department of Women's and Reproductive Health University of Oxford, Women's Centre, John Radcliffe Hospital Oxford UK
- Lorraine O'Driscoll
- School of Pharmacy and Pharmaceutical Sciences Trinity College Dublin Dublin Ireland
- Edit I. Buzas
- Department of Genetics, Cell‐ and Immunobiology Semmelweis University Budapest Hungary
- Cherie Blenkiron
- Faculty of Medical and Health Sciences The University of Auckland Auckland New Zealand
- Benedetta Bussolati
- Department of Molecular Biotechnology and Health Sciences University of Turin Turin Italy
- Houjian Cai
- University of Georgia Athens Georgia USA
- Dolores Di Vizio
- Department of Surgery, Division of Cancer Biology and Therapeutics Cedars‐Sinai Medical Center Los Angeles California USA
- Tom A. P. Driedonks
- Department CDL Research University Medical Center Utrecht Utrecht The Netherlands
- Uta Erdbrügger
- University of Virginia Health System Charlottesville Virginia USA
- Juan M. Falcon‐Perez
- Exosomes Laboratory, Center for Cooperative Research in Biosciences Basque Research and Technology Alliance Derio Spain
- Qing‐Ling Fu
- Otorhinolaryngology Hospital, The First Affiliated Hospital Sun Yat‐sen University Guangzhou China
- Andrew F. Hill
- Institute for Health and Sport Victoria University Melbourne Australia
- Metka Lenassi
- Faculty of Medicine University of Ljubljana Ljubljana Slovenia
- Sai Kiang Lim
- Institute of Molecular and Cell Biology (IMCB) Agency for Science, Technology and Research (A*STAR) Singapore Singapore
- Mỹ G. Mahoney
- Thomas Jefferson University Philadelphia Pennsylvania USA
- Sujata Mohanty
- Stem Cell Facility All India Institute of Medical Sciences New Delhi India
- Andreas Möller
- Chinese University of Hong Kong Hong Kong Hong Kong S.A.R.
- Rienk Nieuwland
- Laboratory of Experimental Clinical Chemistry, Amsterdam University Medical Centers, Location AMC University of Amsterdam Amsterdam The Netherlands
- Takahiro Ochiya
- Tokyo Medical University Tokyo Japan
- Susmita Sahoo
- Icahn School of Medicine at Mount Sinai New York New York USA
- Ana C. Torrecilhas
- Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários, Departamento de Ciências Farmacêuticas, Instituto de Ciências Ambientais, Químicas e Farmacêuticas Universidade Federal de São Paulo (UNIFESP) Campus Diadema Diadema Brazil
- Lei Zheng
- Department of Laboratory Medicine, Nanfang Hospital Southern Medical University Guangzhou China
- Andries Zijlstra
- Department of Pathology Vanderbilt University Medical Center Nashville Tennessee USA
- Sarah Abuelreich
- Department of Molecular Medicine, Beckman Research Institute City of Hope Comprehensive Cancer Center Duarte California USA
- Reem Bagabas
- Department of Molecular Medicine, Beckman Research Institute City of Hope Comprehensive Cancer Center Duarte California USA
- Paolo Bergese
- Department of Molecular and Translational Medicine University of Brescia Brescia Italy
- Esther M. Bridges
- Weatherall Institute of Molecular Medicine University of Oxford Oxford UK
- Marco Brucale
- Consiglio Nazionale delle Ricerche ‐ Istituto per lo Studio dei Materiali Nanostrutturati Bologna Italy
- Dylan Burger
- Kidney Research Centre Ottawa Hopsital Research Institute Ottawa Canada
- Randy P. Carney
- Department of Biomedical Engineering University of California Davis California USA
- Emanuele Cocucci
- Division of Pharmaceutics and Pharmacology, College of Pharmacy The Ohio State University Columbus Ohio USA
- Rossella Crescitelli
- Sahlgrenska Center for Cancer Research, Department of Surgery, Institute of Clinical Sciences Sahlgrenska Academy, University of Gothenburg Gothenburg Sweden
- Edveena Hanser
- Department of Biomedicine University Hospital Basel Basel Switzerland
- Adrian L. Harris
- Department of Oncology University of Oxford Oxford UK
- Norman J. Haughey
- Departments of Neurology and Psychiatry Johns Hopkins University School of Medicine Baltimore Maryland USA
- An Hendrix
- Laboratory of Experimental Cancer Research, Department of Human Structure and Repair Ghent University Ghent Belgium
- Alexander R. Ivanov
- Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology Northeastern University Boston Massachusetts USA
- Tijana Jovanovic‐Talisman
- Department of Cancer Biology and Molecular Medicine, Beckman Research Institute City of Hope Comprehensive Cancer Center Duarte California USA
- Nicole A. Kruh‐Garcia
- Bio‐pharmaceutical Manufacturing and Academic Resource Center (BioMARC) Infectious Disease Research Center, Colorado State University Fort Collins Colorado USA
- Vroniqa Ku'ulei‐Lyn Faustino
- Department of Molecular Medicine, Beckman Research Institute City of Hope Comprehensive Cancer Center Duarte California USA
- Diego Kyburz
- Department of Biomedicine University of Basel Basel Switzerland
- Cecilia Lässer
- Krefting Research Centre, Department of Internal Medicine and Clinical Nutrition Institute of Medicine at Sahlgrenska Academy, University of Gothenburg Gothenburg Sweden
- Kathleen M. Lennon
- Department of Molecular Medicine, Beckman Research Institute City of Hope Comprehensive Cancer Center Duarte California USA
- Jan Lötvall
- Krefting Research Centre, Institute of Medicine at Sahlgrenska Academy University of Gothenburg Gothenburg Sweden
- Adam L. Maddox
- Department of Molecular Medicine, Beckman Research Institute City of Hope Comprehensive Cancer Center Duarte California USA
- Elena S. Martens‐Uzunova
- Erasmus MC Cancer Institute University Medical Center Rotterdam, Department of Urology Rotterdam The Netherlands
- Rachel R. Mizenko
- Department of Biomedical Engineering University of California Davis California USA
- Lauren A. Newman
- College of Medicine and Public Health Flinders University Adelaide Australia
- Andrea Ridolfi
- Department of Physics and Astronomy, and LaserLaB Amsterdam Vrije Universiteit Amsterdam Amsterdam The Netherlands
- Eva Rohde
- Department of Transfusion Medicine, University Hospital Salzburger Landeskliniken GmbH of Paracelsus Medical University Salzburg Austria
- Tatu Rojalin
- Department of Biomedical Engineering University of California Davis California USA
- Andrew Rowland
- College of Medicine and Public Health Flinders University Adelaide Australia
- Andras Saftics
- Department of Molecular Medicine, Beckman Research Institute City of Hope Comprehensive Cancer Center Duarte California USA
- Ursula S. Sandau
- Department of Anesthesiology & Perioperative Medicine Oregon Health & Science University Portland Oregon USA
- Julie A. Saugstad
- Department of Anesthesiology & Perioperative Medicine Oregon Health & Science University Portland Oregon USA
- Faezeh Shekari
- Department of Stem Cells and Developmental Biology, Cell Science Research Center Royan Institute for Stem Cell Biology and Technology, ACECR Tehran Iran
- Simon Swift
- Waipapa Taumata Rau University of Auckland Auckland New Zealand
- Dmitry Ter‐Ovanesyan
- Wyss Institute for Biologically Inspired Engineering Harvard University Boston Massachusetts USA
- Juan P. Tosar
- Universidad de la República Montevideo Uruguay
- Zivile Useckaite
- College of Medicine and Public Health Flinders University Adelaide Australia
- Francesco Valle
- Consiglio Nazionale delle Ricerche ‐ Istituto per lo Studio dei Materiali Nanostrutturati Bologna Italy
- Zoltan Varga
- Biological Nanochemistry Research Group Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences Budapest Hungary
- Edwin van derPol
- Biomedical Engineering and Physics, Amsterdam UMC, location AMC University of Amsterdam Amsterdam The Netherlands
- Martijn J. C. vanHerwijnen
- Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine Utrecht University Utrecht The Netherlands
- Marca H. M. Wauben
- Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine Utrecht University Utrecht The Netherlands
- Ann M. Wehman
- University of Denver Denver USA
- Sarah Williams
- International Society for Extracellular Vesicles
- Andrea Zendrini
- Department of Molecular and Translational Medicine University of Brescia Brescia Italy
- Alan J. Zimmerman
- Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology Northeastern University Boston Massachusetts USA
- MISEV Consortium
- Clotilde Théry
- Institut Curie, INSERM U932 PSL University Paris France
- Kenneth W. Witwer
- Department of Molecular and Comparative Pathobiology Johns Hopkins University School of Medicine Baltimore Maryland USA
- DOI
- https://doi.org/10.1002/jev2.12404
- Journal volume & issue
-
Vol. 13,
no. 2
pp. n/a – n/a
Abstract
Abstract Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year‐on‐year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non‐vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.
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