Journal of Lipid Research (Jun 1988)

Separation of apolipoprotein B species by agarose-acrylamide gel electrophoresis.

  • C Gabelli,
  • D G Stark,
  • R E Gregg,
  • H B Brewer, Jr

DOI
https://doi.org/10.1016/s0022-2275(20)38827-1
Journal volume & issue
Vol. 27, no. 4
pp. 457 – 460

Abstract

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Human apolipoprotein (apo) B has been recognized to exist in two different forms designated apoB-100 and apoB-48. The two apoB forms are usually separated by NaDodSO4 gel electrophoresis with a low percentage polyacrylamide gel in a tube gel apparatus. However, the matrix of this low percentage gel is relatively weak, and one can separate the two forms of apoB in a slab gel apparatus only if one utilizes a gradient polyacrylamide gel or a higher percentage polyacrylamide gel which results in a poorer separation of the protein bands. We have developed an agarose-acrylamide gel electrophoretic method to separate the two major apoB forms. The gel is a mixture of 0.5% agarose and 2% acrylamide. The agarose-acrylamide method is fast, has the advantage of being able to be used on an analytical or preparative scale in a vertical slab gel apparatus, and the gel is of sufficient strength to be used in immunoblotting and/or radioautography.