Effect of fluorophenylalanine on indole-3-acetic acid levels in Aveaa coleoptiles

Abstract

The effect of the amino acid analogue D,L-p-fluorophenylalanine on indole-3-acetic acid levels in Avena has been examined. Previous studies have established that D,L-p-fluorophenylalanine promotes elongation, lowers phenolic levels and depresses auxin oxidase activity of etiolated Avena coleoptiles. This study employs an enzyme immunoassay to measure endogenous indole-3-acetic acid concentrations in coleoptile apices. These data demonstrate that treatment of Avena coleoptiles with D,L-p-fluorophenylalanine results in altered auxin levels and help clarify the mechanism of D,L-p-fluorophenylalanine action in Avena

Keywords

• Avena coleoptile
• enzyme immunoassay
• fluorophenylalanine
• indole-3-acetic acid
• phenolic metabolism