CELLULOLYTIC ACTIVITY OF STRAIN OF THE FUNGUS ACREMONIUM SP. 502 PICKED UP FROM AFFECTED CUCUMBER PLANTS

Abstract

The aim of the work was to study the cellulase activity of the strain Acremonium sp. 502 depending on medium pH, as a determinant of its penetration into the plant. Methods. The strain Acremonium sp. 502 was cultivated on wort agar. The optimal pH was detected by growth diameter of the mold colony. We used filter paper, microcrystalline cellulose, carboxymethyl cellulose and cellobiose to fix cellulase, exoglucanase, endoglucanase and β-glucosidase activity. The amount of reducing sugars was determined by Somogyi-Nelson method. Results. It was investigated the dynamics of cellulase activity at different pH values of culture medium of the mold Acremonium sp. 502 isolated from stroked cucumber plants. Maximal enzyme activity values were detected after 6 weeks of mold cultivating at pH 8.5. Herewith the general cellulase activity was 1.95 u/ml, exoglucanase – 3.23, endoglucanase – 2.85, and β-glucosidase – 2.39 u/ml, which evidences in favor of the enzymatic mechanism of mold penetration into the plant tissues and the subsequent development of the disease. Conclusions. It was established that Acremonium sp. 502 is capable to synthesize exo-, endoglucanase and β-glucosidase. The most cellulolytic activity was detected after 6 weeks of mold cultivating at pH 8.5. It can be assumed that formation of cellulase enzymes with the mold is necessary for its penetrating in plant followed by the development of the disease.

Keywords

• загальна целюлазна
• екзоглюканазна
• ендоглюканазна і β-глюкозидазна активності
• acremonium sp