Virus resistance obtained in transgenic tobacco and rice by RNA interference using promoters with distinct activity

C. Zhang; Y. Song; F. Jiang; G. Li; Y. Jiang; C. Zhu; F. Wen

doi:10.1007/s10535-012-0117-z

Biologia Plantarum (Dec 2012)

Virus resistance obtained in transgenic tobacco and rice by RNA interference using promoters with distinct activity

C. Zhang,
Y. Song,
F. Jiang,
G. Li,
Y. Jiang,
C. Zhu,
F. Wen

Affiliations

C. Zhang: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, P.R. China
Y. Song: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, P.R. China
F. Jiang: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, P.R. China
G. Li: Rice Research Institute, Shandong Academy of Agricultural Sciences, Ji'nan, Shandong, P.R. China
Y. Jiang: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, P.R. China
C. Zhu: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, P.R. China
F. Wen: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, P.R. China

DOI: https://doi.org/10.1007/s10535-012-0117-z
Journal volume & issue: Vol. 56, no. 4
pp. 742 – 748

Abstract

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To induce virus resistance in tobacco and rice we constructed hairpin RNA expression system harbouring inverted repeat fragments of coat protein cDNA of Potato virus Y (PVY) or Rice stripe virus (RSV). These structures were driven by three promoters [cauliflower mosaic virus 35S (CaMV 35S), polyubiqutin gene of maize (Ubi), and Pharbitis nil leucine zipper gene (PNZIP)] which have different tissue-specific activity. PVY resistance ratios were 65.18, 24.33 and 83.54 % in transgenic tobacco plants harboring p35S-PVY, pUbi-PVY and pPNZIP-PVY. RSV resistance was 16.21, 28.61 and 29.33 % in transgenic rice plants harboring p35S-RSV, pUbi-RSV and pPNZIP-RSV. Northern blotting and GUS assay demonstrated that virus resistance levels were related to promoter activity. Therefore, choice of the more effective and tissue-specific promoter to reinforce transcription of hpRNAs will favour the cultivation of highly virusresistant transgenic plants.

Published in Biologia Plantarum

ISSN: 1573-8264 (Online)
Publisher: Institute of Experimental Botany of the Czech Academy of Sciences
Country of publisher: Czechia
LCC subjects: Science: Biology (General); Science: Botany: Plant ecology
Website: https://bp.ueb.cas.cz/

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