Arabian Journal of Chemistry (Nov 2017)

A simple reversed phase high-performance liquid chromatography (HPLC) method for determination of in situ gelling curcumin-loaded liquid crystals in in vitro performance tests

  • Bruno Fonseca-Santos,
  • Maria Palmira Daflon Gremião,
  • Marlus Chorilli

DOI
https://doi.org/10.1016/j.arabjc.2016.01.014
Journal volume & issue
Vol. 10, no. 7
pp. 1029 – 1037

Abstract

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A simple, rapid, and sensitive analytical procedure has been developed and validated for the in vitro measurement of curcumin in samples from mucosae retention studies. Curcumin was analyzed by HPLC using a C18 column with UV detection at 425 nm. The mobile phase was acetonitrile and water (50:50 v/v) acidified with 2% acetic acid at a flow rate of 1.2 mL min−1. The curve range was linear for the receptor solution concentration range 0.5–75 μg mL−1. The specificity showed no interference with the biological matrix and excipients of the acceptor media. Intra and inter-day accuracy, and precision values were lower than 5% and were not statically different (P < 0.05). Recoveries ranged from 99.7% to 108%. The limits of detection and quantitation were 11.61 and 500 ng mL−1, respectively. The method is adequate to assay curcumin from esophageal porcine samples, enabling the determination of penetration profiles for in situ gelling curcumin-loaded liquid crystals by in vitro studies, and fulfilled the requirements for reliability and feasibility for application to the quantitative analysis of curcumin in porcine esophageal mucosae.

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