Complete and cooperative in vitro assembly of computationally designed self-assembling protein nanomaterials

Adam J. Wargacki; Tobias P. Wörner; Michiel van de Waterbeemd; Daniel Ellis; Albert J. R. Heck; Neil P. King

doi:10.1038/s41467-021-21251-y

Nature Communications (Feb 2021)

Complete and cooperative in vitro assembly of computationally designed self-assembling protein nanomaterials

Adam J. Wargacki,
Tobias P. Wörner,
Michiel van de Waterbeemd,
Daniel Ellis,
Albert J. R. Heck,
Neil P. King

Affiliations

Adam J. Wargacki: Department of Biochemistry, University of Washington
Tobias P. Wörner: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University
Michiel van de Waterbeemd: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University
Daniel Ellis: Department of Biochemistry, University of Washington
Albert J. R. Heck: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University
Neil P. King: Department of Biochemistry, University of Washington

DOI: https://doi.org/10.1038/s41467-021-21251-y
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 14

Abstract

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Recent advances in computational methods have enabled the predictive design of self-assembling protein nanomaterials with atomic-level accuracy. Here authors investigate the assembly of two computationally designed, 120-subunit icosahedral complexes and find that assembly of each material from its two constituent protein building blocks was highly cooperative.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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